de Cássia Spacki Kamila, Novi Danielly Maria Paixão, de Oliveira-Junior Verci Alves, Durigon Daniele Cocco, Fraga Fernanda Cristina, Dos Santos Luís Felipe Oliva, Helm Cristiane Vieira, de Lima Edson Alves, Peralta Rosely Aparecida, de Fátima Peralta Muniz Moreira Regina, Corrêa Rúbia Carvalho Gomes, Bracht Adelar, Peralta Rosane Marina
Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá 87020-900, Brazil.
Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis 88040-900, Brazil.
Plants (Basel). 2023 Jul 31;12(15):2824. doi: 10.3390/plants12152824.
The white-rot fungus was used for biological pretreatment of peach palm () lignocellulosic wastes. Non-treated and treated inner sheaths and peel were submitted to hydrolysis using a commercial cellulase preparation from . The amounts of total reducing sugars and glucose obtained from the 30 d-pretreated inner sheaths were seven and five times higher, respectively, than those obtained from the inner sheaths without pretreatment. No such improvement was found, however, in the pretreated peels. Scanning electronic microscopy of the lignocellulosic fibers was performed to verify the structural changes caused by the biological pretreatments. Upon the biological pretreatment, the lignocellulosic structures of the inner sheaths were substantially modified, making them less ordered. The main features of the modifications were the detachment of the fibers, cell wall collapse and, in several cases, the formation of pores in the cell wall surfaces. The peel lignocellulosic fibers showed more ordered fibrils and no modification was observed after pre-treatment. In conclusion, a seven-fold increase in the enzymatic saccharification of the inner sheath was observed after pre-treatment, while no improvement in enzymatic saccharification was observed in the peel.
白腐真菌被用于对桃棕()木质纤维素废料进行生物预处理。将未经处理和经过处理的内鞘和果皮使用来自的商业纤维素酶制剂进行水解。从经过30天预处理的内鞘中获得的总还原糖和葡萄糖量分别比未经预处理的内鞘高出7倍和5倍。然而,在经过预处理的果皮中未发现这种改善。对木质纤维素纤维进行扫描电子显微镜检查,以验证生物预处理引起的结构变化。经过生物预处理后,内鞘的木质纤维素结构发生了显著改变,使其有序性降低。这些改变的主要特征是纤维分离、细胞壁塌陷,并且在几种情况下,细胞壁表面形成了孔隙。果皮木质纤维素纤维显示出更有序的原纤维,预处理后未观察到变化。总之,预处理后内鞘的酶促糖化增加了7倍,而果皮的酶促糖化未得到改善。