Sonka J, Günther A, Volm M
Cancer Chemother Pharmacol. 1986;18(1):83-5. doi: 10.1007/BF00253071.
Adriamycin-resistant cell lines of the sarcoma 180 of mouse reveal, besides the known resistance mechanism, a decrease in cellular autolysis compared with the original line. This decrease has been demonstrated by comparative analysis of cell-free supernatants isolated from 7-day-old sensitive and resistant ascites tumors in mice. In order to demonstrate these changes we isolated and quantified the DNA, detecting higher amounts within the supernatants of resistant lines. Furthermore, unspecific DNA-cleaving activity within raw homogenates of the cells is substantially lower in the resistant lines. Residual parts of chromatin may trap the drug and in this way lower its effective concentration. However, the results may also reflect changes in enzymatic complement playing a hitherto unknown role within living cells.
小鼠肉瘤180阿霉素耐药细胞系除了已知的耐药机制外，与原始细胞系相比，细胞自溶作用有所降低。通过对从小鼠7日龄敏感和耐药腹水瘤中分离的无细胞上清液进行比较分析，证实了这种降低。为了证明这些变化，我们分离并定量了DNA，检测到耐药细胞系上清液中的DNA含量更高。此外，耐药细胞系中细胞粗匀浆内的非特异性DNA切割活性明显较低。染色质的残留部分可能会捕获药物，从而降低其有效浓度。然而，这些结果也可能反映了酶成分的变化，这种变化在活细胞中发挥着迄今未知的作用。
