Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, P.O. Box 61413, Abha, 9088, Saudi Arabia.
Department of microbiology and botany, Faculty of Science, Zagazig university, Egypt.
Cell Mol Biol (Noisy-le-grand). 2023 May 31;69(5):6-11. doi: 10.14715/cmb/2023.69.5.2.
Multidrug-resistant (MDR) bacteria are one of the major public health threats facing humanity. Infections with MDR strains are difficult or impossible to treat with standard antibiotics leading to severe illnesses and even deaths. The spread of MDR bacteria has necessitated the search for alternative approaches that tackle MDR pathogens. Natural plants can be utilized as alternative therapeutic agents against the rise of MDR bacteria. In this study, we aimed to assess the antimicrobial activity of pomegranate peel extracts (PPE) against MDR clinical isolates. A total of 9 clinical isolates (8 MDR and 1 non-MDR clinical isolates) were collected and examined for their susceptibility to PPE. Using the zone of inhibition assay, 4 isolates (S. aureus, three MRSA isolates, Vancomycin-resistant Enterococci (VRE), and Acinetobacter baumannii) were sensitive to PPE. In Broth assay, 4 mg/ml PPE significantly reduced the growth (S. aureus, three MRSA isolates, Vancomycin-resistant Enterococci (VRE), and Acinetobacter baumannii), while 40 mg/ml PPE either significantly reduced or completely inhibited the growth of the isolates. The minimum bactericidal concentration (MBC) of PPE against S. aureus and MRSA-88 was 10 mg/ml. This study showed the potential of PPE as an alternative compound for treating infections caused by PPE-sensitive MDR bacteria.
耐多药 (MDR) 细菌是人类面临的主要公共卫生威胁之一。感染 MDR 菌株用标准抗生素难以治疗甚至无法治疗，导致严重疾病甚至死亡。MDR 细菌的传播使得必须寻找其他方法来解决 MDR 病原体。天然植物可用作治疗 MDR 细菌上升的替代治疗剂。在这项研究中，我们旨在评估石榴皮提取物 (PPE) 对 MDR 临床分离株的抗菌活性。共收集了 9 株临床分离株（8 株 MDR 和 1 株非 MDR 临床分离株）并检查它们对 PPE 的敏感性。使用抑菌圈试验，4 株分离株（金黄色葡萄球菌、3 株耐甲氧西林金黄色葡萄球菌、万古霉素耐药肠球菌 (VRE) 和鲍曼不动杆菌）对 PPE 敏感。在肉汤试验中，4 mg/ml PPE 显著降低了 4 株分离株（金黄色葡萄球菌、3 株耐甲氧西林金黄色葡萄球菌、万古霉素耐药肠球菌 (VRE) 和鲍曼不动杆菌）的生长，而 40 mg/ml PPE 要么显著降低要么完全抑制了分离株的生长。PPE 对金黄色葡萄球菌和 MRSA-88 的最低杀菌浓度 (MBC) 为 10 mg/ml。这项研究表明 PPE 有潜力作为治疗 PPE 敏感 MDR 细菌引起的感染的替代化合物。
