College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.
Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, Luoyang, 471023, China.
Mol Biol Rep. 2023 Oct;50(10):8259-8270. doi: 10.1007/s11033-023-08727-4. Epub 2023 Aug 12.
The ClaH3K4s and ClaH3K27s gene families are subfamilies of the SET family, each with a highly conserved SET structure domain and a PHD structural domain. Both participate in histone protein methylation, which affects the chromosome structure and gene expression, and is essential for fruit growth and development.
In order to demonstrate the structure and expression characteristics of ClaH3K4s and ClaH3K27s in watermelon, members of the watermelon H3K4 and H3K27 gene families were identified, and their chromosomal localization, gene structure, and protein structural domains were analyzed. The phylogeny and covariance of the gene families with other species were subsequently determined, and the expression profiles were obtained by performing RNA-Seq and qRT-PCR. The watermelon genome had five H3K4 genes with 3207-8043 bp nucleotide sequence lengths and four H3K27 genes with a 1107-5499 bp nucleotide sequence. Synteny analysis revealed the close relationship between watermelon and cucumber, with the majority of members displaying a one-to-one covariance. Approximately half of the 'Hua-Jing 13 watermelon' ClaH3K4s and ClaH3K27s genes were expressed more in the late fruit development stages, while the changes were minimal for the remaining half. H3K4-2 expression was observed to be slightly greater on day 21 compared to other periods. Moreover, ClaH3K27-1 and ClaH3K27-2 were hardly expressed throughout the developing period, and ClaH3K27-4 exhibited the highest expression.
These results serve as a basis for further functional characterization of the H3K4 and H3K27 genes in the fruit development of watermelon.
ClaH3K4s 和 ClaH3K27s 基因家族是 SET 家族的亚家族，每个家族都具有高度保守的 SET 结构域和 PHD 结构域。它们都参与组蛋白蛋白甲基化，影响染色体结构和基因表达，对果实生长发育至关重要。
为了阐明西瓜 ClaH3K4s 和 ClaH3K27s 的结构和表达特征，鉴定了西瓜 H3K4 和 H3K27 基因家族的成员，并对其染色体定位、基因结构和蛋白结构域进行了分析。随后，确定了与其他物种的基因家族的系统发育和共线性，并通过 RNA-Seq 和 qRT-PCR 获得了表达谱。西瓜基因组有 5 个 H3K4 基因，核苷酸序列长度为 3207-8043bp，4 个 H3K27 基因，核苷酸序列长度为 1107-5499bp。同线性分析表明，西瓜与黄瓜关系密切，大多数成员呈一对一共线性。‘华晶 13 号西瓜’ClaH3K4s 和 ClaH3K27s 基因的近一半在果实发育后期表达较高，另一半变化较小。H3K4-2 的表达在第 21 天比其他时期略有增加。此外，ClaH3K27-1 和 ClaH3K27-2 在整个发育过程中几乎不表达，ClaH3K27-4 表达量最高。
这些结果为进一步研究西瓜果实发育过程中 H3K4 和 H3K27 基因的功能提供了基础。
