Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
School of Medicine, College of Medical School, Kaohsiung Medical University, Kaohsiung, Taiwan.
Kaohsiung J Med Sci. 2023 Nov;39(11):1129-1134. doi: 10.1002/kjm2.12742. Epub 2023 Aug 13.
In order to accurately monitor graft immunology, we have developed a method for performing intestine and abdominal wall transplantation heterotopically in miniature swine. The procedure consisted of simultaneous segmental terminal ileum and full-thickness abdominal wall transplantation in Lanyu miniature swine, with the intestinal and the abdominal wall grafts being placed on the recipient's bilateral rear flank. Five transplantations were technically successful. One animal died on the first post-transplant day due to anesthesia-related issues, three abdominal wall and four intestinal grafts survived, while one abdominal wall graft failed due to vascular thrombosis. Acute cellular rejection (ACR) of the intestinal graft could occur preceding, simultaneously with or following ACR of the abdominal wall graft. Our experimental model demonstrates the technical feasibility of heterotopic intestine and abdominal wall transplantation in miniature swine without grafting in gastrointestinal continuity. This model could be suitable for further studies of graft immunology.
为了准确监测移植物免疫，我们开发了一种在小型猪中进行肠和腹壁异位移植的方法。该方法包括在兰屿小型猪中同时进行节段性末端回肠和全层腹壁移植，将肠和腹壁移植物放置在受者双侧后肋部。5 例手术均获得成功。1 只动物因麻醉相关问题于移植后第 1 天死亡，3 个腹壁和 4 个肠移植物存活，1 个腹壁移植物因血管血栓形成而失败。肠移植物的急性细胞排斥反应（ACR）可发生在腹壁移植物 ACR 之前、同时或之后。我们的实验模型证明了在不进行胃肠道连续性移植的情况下，在小型猪中进行异位肠和腹壁移植的技术可行性。该模型可能适合进一步研究移植物免疫。
