Applied Environmental Microbial Biotechnology Laboratory, Department of Environmental Science, Nagaland University, Lumami, Zunheboto 798627, Nagaland, India.
Applied Environmental Microbial Biotechnology Laboratory, Department of Environmental Science, Nagaland University, Lumami, Zunheboto 798627, Nagaland, India.
Int J Biol Macromol. 2023 Nov 1;251:126309. doi: 10.1016/j.ijbiomac.2023.126309. Epub 2023 Aug 12.
In the present study, bacterial nanocellulose/graphene oxide nano-biocomposites (BNC-GO-NBCs) were fabricated by Komagataeibacter saccharivorans NUWB1 using an in-situ method involving three time-dependent approaches. Physicochemical studies showed that the chosen dried BNC-GO-NBC possessed a three-dimensional interconnected porous structure of BNC with GO layers embedded within the BNC fibrils. BNC-GO-NBC had a crystallinity index of 74.21 %, higher thermostability up to 380 °C and could withstand a tensile load of 84.72 MPa. N adsorption-desorption isotherm of the BNC-GO-NBC was found to be of type IV, suggesting a mesoporous type structure with a total pore volume and surface area of 6.232e-04 cc g and 10.498 m. BNC-GO-NBC exhibited remarkable adsorption capacity for two cationic dyes, Rhodamine B (RhB) and Acridine Orange (AO), and the adsorption data conformed well to the Langmuir isotherm (R = 0.99) and pseudo-second-order model. Thermodynamic studies indicated that the adsorption process was spontaneous and endothermic. Additionally, the BNC-GO-NBC displayed the potential for regeneration, with the ability to be recycled up to five times. Further, the antibacterial activity, cell cytotoxicity and oxidative stress assays of the BNC-GO-NBC revealed its non-cytotoxic nature. The findings of the present investigation evidently suggest the potentiality of BNC-GO-NBC in the application of dye adsorption and other environmental applications.
在本研究中，Komagataeibacter saccharivorans NUWB1 通过原位法制备了细菌纳米纤维素/氧化石墨烯纳米生物复合材料（BNC-GO-NBCs），该方法涉及三个时间依赖性方法。物理化学研究表明，所选的干燥 BNC-GO-NBC 具有 BNC 的三维相互连接的多孔结构，GO 层嵌入在 BNC 原纤维内。BNC-GO-NBC 的结晶度指数为 74.21%，热稳定性更高，可达 380°C，并且能够承受 84.72 MPa 的拉伸载荷。发现 BNC-GO-NBC 的氮气吸附-解吸等温线为 IV 型，表明具有中孔类型结构，总孔体积和表面积分别为 6.232e-04 cc g 和 10.498 m。BNC-GO-NBC 对两种阳离子染料，罗丹明 B（RhB）和吖啶橙（AO）具有显著的吸附能力，吸附数据符合朗缪尔等温线（R = 0.99）和拟二级模型。热力学研究表明，吸附过程是自发的和吸热的。此外，BNC-GO-NBC 显示出再生的潜力，能够循环使用多达五次。此外，BNC-GO-NBC 的抗菌活性、细胞毒性和氧化应激测定表明其无细胞毒性。本研究的结果明显表明了 BNC-GO-NBC 在染料吸附和其他环境应用中的应用潜力。
