State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi 530004, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi 530004, China.
Plant Sci. 2023 Oct;335:111826. doi: 10.1016/j.plantsci.2023.111826. Epub 2023 Aug 11.
CONSTANS (CO) is the key gene in the photoperiodic pathway that regulates flowering in plants. In this paper, a CONSTANS-like 14A (COL14A) gene was obtained from mango, and its expression patterns and functions were characterized. Sequence analysis shows that MiCOL14A-JH has an additional A base, which leads to code shifting in subsequent coding boxes and loss of the CCT domain. The MiCOL14A-JH and MiCOL14A-GQ genes both belonged to group Ⅲ of the CO/COL gene family. Analysis of tissue expression patterns showed that MiCOL14A was expressed in all tissues, with the highest expression in the leaves of seedling, followed by lower expression levels in the flowers and stems of adult leaves. However, there was no significant difference between different mango varieties. At different development stages of flowering, the expression level of MiCOL14A-GQ was the highest in the leaves before floral induction period, and the lowest at flowering stage, while the highest expression level of MiCOL14A-JH appeared in the leaves at flowering stage. The transgenic functional analysis showed that both MiCOL14A-GQ and MiCOL14A-JH induced delayed flowering of transgenic Arabidopsis. In addition, MiCOL14A-JH enhanced the resistance of transgenic Arabidopsis to drought stress, while MiCOL14A-GQ increased the sensitivity of transgenic Arabidopsis to salt stress. Further proteinprotein interaction analysis showed that MiCOL14A-JH directly interacted with MYB30-INTERACTING E3 LIGASE 1 (MiMIEL1), CBL-interacting protein kinase 9 (MiCIPK9) and zinc-finger protein 4 (MiZFP4), but MiCOL14A-GQ could not interact with these three stress-related proteins. Together, our results demonstrated that MiCOL14A-JH and MiCOL14A-GQ not only regulate flowering but also play a role in the abiotic stress response in mango, and the lack of the CCT domain affects the proteinprotein interaction, thus affecting the gene response to stress. The insertion of an A base can provide a possible detection site for mango resistance breeding.
CONSTANS（CO）是调控植物开花的光周期途径中的关键基因。本文从芒果中获得了一个CONSTANS-like 14A（COL14A）基因，并对其表达模式和功能进行了表征。序列分析表明，MiCOL14A-JH 有一个额外的 A 碱基，导致后续编码框的移码，并丢失 CCT 结构域。MiCOL14A-JH 和 MiCOL14A-GQ 基因均属于 CO/COL 基因家族的第Ⅲ组。组织表达模式分析表明，MiCOL14A 在所有组织中均有表达，在幼苗叶片中表达量最高，其次是成年叶片的花和茎中表达量较低。然而，不同芒果品种之间没有显著差异。在开花不同发育阶段，MiCOL14A-GQ 在花诱导前叶片中的表达水平最高，在开花期最低，而 MiCOL14A-JH 的最高表达水平出现在开花期叶片中。转基因功能分析表明，MiCOL14A-GQ 和 MiCOL14A-JH 均诱导转基因拟南芥开花延迟。此外，MiCOL14A-JH 增强了转基因拟南芥对干旱胁迫的抗性，而 MiCOL14A-GQ 增加了转基因拟南芥对盐胁迫的敏感性。进一步的蛋白-蛋白相互作用分析表明，MiCOL14A-JH 直接与 MYB30-INTERACTING E3 LIGASE 1（MiMIEL1）、CBL-interacting protein kinase 9（MiCIPK9）和锌指蛋白 4（MiZFP4）相互作用，但 MiCOL14A-GQ 不能与这三个与应激相关的蛋白相互作用。综上所述，我们的研究结果表明，MiCOL14A-JH 和 MiCOL14A-GQ 不仅调控开花，还在芒果的非生物胁迫响应中发挥作用，且缺乏 CCT 结构域会影响蛋白-蛋白相互作用，从而影响基因对胁迫的响应。A 碱基的插入为芒果抗性育种提供了一个可能的检测位点。
