O'Day D M, Akrabawi P L, Head W S, Ratner H B
Am J Ophthalmol. 1979 May;87(5):688-93. doi: 10.1016/0002-9394(79)90305-2.
In laboratory experience with a heterogenous group of 26 human ocular fungal isolates, brain-heart infusion broth proved to be the most useful medium for isolation. Although Candida and Fusarium species grew out within four days of inoculation, one fourth of the cultures did not become positive until 14 to 19 days had elapsed. In an animal model of endophthalmitis due to F. solani infection, brain-heart infusion broth again was the most useful. The highly nutritious media used for fungal isolation are prone to contamination by organisms that are difficult to distinguish from true pathogens. Sham culture studies demonstrated that this contamination can easily occur during the process of sampling the lesion and inoculating the media.
在对26株人眼真菌分离株组成的异质组进行的实验室实验中，脑心浸液肉汤被证明是最有利于分离的培养基。尽管念珠菌属和镰刀菌属菌种在接种后四天内就生长出来了，但四分之一的培养物直到14至19天后才呈阳性。在茄病镰刀菌感染所致眼内炎的动物模型中，脑心浸液肉汤同样最为适用。用于真菌分离的高营养培养基容易被难以与真正病原体区分的生物体污染。模拟培养研究表明，这种污染在对病变进行采样和接种培养基的过程中很容易发生。
