González-Arreola Rosa María, García-Romero Adriana, Magaña-Torres María Teresa, González-García Juan Ramón
Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, CIBO-IMSS, Sierra Mojada #800, Colonia Independencia, CP 44340, Guadalajara, Jalisco, Mexico.
Mol Cytogenet. 2023 Aug 13;16(1):19. doi: 10.1186/s13039-023-00652-2.
High expression of the Cytokine Receptor-Like Factor 2 (CRLF2) gene has been observed in patients with acute lymphoblastic leukemia BCR-ABL1-like subtype. Currently, there is no commercial system available for the direct detection of the IGH::CRLF2 fusion by fluorescent in situ hybridization (FISH), as there are for many other leukemia-related gene fusions. In an effort to verify the IGH::CRLF2 fusion, some researchers prepare home-grown FISH probes from bacterial artificial chromosome clones flanking the IGH and CRLF2 genes, which is the best alternative to confirm the fusion, however difficult to reproduce in most cytogenetic laboratories.
For the direct observation of the IGH::CRLF2 gene fusion we designed a methodological approach requiring the two commercially available IGH and CRLF2 break-apart probes.
Our methodological approach allows direct visualization of the IGH::CRLF2 gene fusion and has the potential to be used for identification of other gene fusions.
在B细胞急性淋巴细胞白血病BCR-ABL1样亚型患者中观察到细胞因子受体样因子2（CRLF2）基因的高表达。目前，尚无用于通过荧光原位杂交（FISH）直接检测IGH::CRLF2融合的商业系统，而许多其他白血病相关基因融合则有。为了验证IGH::CRLF2融合，一些研究人员从IGH和CRLF2基因侧翼的细菌人工染色体克隆中制备自制的FISH探针，这是确认融合的最佳替代方法，但在大多数细胞遗传学实验室中难以重复。
为了直接观察IGH::CRLF2基因融合，我们设计了一种方法，需要两种市售的IGH和CRLF2断裂探针。
我们的方法允许直接可视化IGH::CRLF2基因融合，并且有可能用于鉴定其他基因融合。
