Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Colloids Surf B Biointerfaces. 2023 Oct;230:113502. doi: 10.1016/j.colsurfb.2023.113502. Epub 2023 Aug 7.
Basic fibroblast growth factor (bFGF) is a therapeutic protein that can enhance angiogenesis, wound healing, and tissue regeneration; however, it is extremely unstable even under a normal physiological environment. Biocompatible calcium phosphate (CaP) nanoparticles (NPs) co-immobilizing bFGF, heparin, and ferucarbotran would be useful as a multifunctional delivery carrier of bFGF. In this study, such NPs were successfully fabricated by a coprecipitation process, using a labile supersaturated CaP solution containing bFGF, heparin, and ferucarbotran. The NPs showed relatively high negative zeta potential (-12 mV) because of the negatively charged heparin, which enabled their stable dispersion in water. The hydrodynamic diameter of the NPs was around 200 nm. Immunoreactive bFGF was released from the NPs in an acellular medium dose-dependently. The NPs promoted proliferation of baby hamster kidney fibroblasts (BHK-21 cells) and mouse osteoblastic MC3T3-E1 cells at a certain dose range, although they inhibited proliferation of rat pheochromocytoma (PC-12) cells. These results demonstrated that the effect of the NPs on cell proliferation was dependent on the cell type and dose, the details of which should be investigated in a future study.
碱性成纤维细胞生长因子（bFGF）是一种治疗性蛋白，可促进血管生成、伤口愈合和组织再生；然而，即使在正常生理环境下，它也极其不稳定。将碱性成纤维细胞生长因子（bFGF）、肝素和 Ferucarbotran 共固定在生物相容性的磷酸钙（CaP）纳米颗粒（NPs）上，可作为 bFGF 的多功能递药载体。在这项研究中，使用含有 bFGF、肝素和 Ferucarbotran 的不稳定过饱和 CaP 溶液，通过共沉淀法成功制备了此类 NPs。由于带负电荷的肝素，NPs 具有相对较高的负 zeta 电位（-12 mV），从而能够在水中稳定分散。NPs 的水动力直径约为 200nm。在无细胞培养基中，NPs 中结合的 bFGF 呈剂量依赖性释放。NPs 在一定剂量范围内促进仓鼠肾成纤维细胞（BHK-21 细胞）和小鼠成骨细胞 MC3T3-E1 细胞的增殖，尽管它们抑制大鼠嗜铬细胞瘤（PC-12 细胞）的增殖。这些结果表明，NPs 对细胞增殖的影响取决于细胞类型和剂量，这在未来的研究中需要进一步研究。
