大鼠和鳟鱼肝中多萜醇及磷酸多萜醇的分离与定量测定。

Separation and quantitative determination of dolichol and dolichyl phosphate in rat and trout liver.

作者信息

Bizri M, Bouhours J F, Bizri Y, Got R

出版信息

Comp Biochem Physiol B. 1986;84(4):565-9. doi: 10.1016/0305-0491(86)90124-0.

Abstract

The dolichol concentrations in rat and trout liver were found respectively to be 50-59 and 16-21 micrograms/g using three experimental methods: densitometric scanning of thin-layer plates, colorimetric assay and HPLC analysis. By HPLC of benzoylated dolichols, the distribution of the dolichols according to the number of their isoprene residues, was determined in rat and trout liver. The major component was dolichol -18 in rat and dolichol -19 in trout liver. Dolichyl phosphate concentrations were found to be 6-7 micrograms/g of rat liver and 8-9 micrograms/g of trout liver by densitometric scanning of thin-layer plates.

摘要

采用三种实验方法，即薄层板光密度扫描、比色法和高效液相色谱分析，分别测定大鼠和鳟鱼肝脏中的多萜醇浓度，结果发现大鼠肝脏中多萜醇浓度为50 - 59微克/克，鳟鱼肝脏中为16 - 21微克/克。通过对苯甲酰化多萜醇进行高效液相色谱分析，确定了大鼠和鳟鱼肝脏中多萜醇按异戊二烯残基数量的分布情况。大鼠肝脏中的主要成分是多萜醇-18，鳟鱼肝脏中的主要成分是多萜醇-19。通过薄层板光密度扫描发现，大鼠肝脏中磷酸多萜醇浓度为6 - 7微克/克，鳟鱼肝脏中为8 - 9微克/克。