Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Chemistry Department, Faculty of Pharmacy, October 6 University, 6th of October City, Egypt.
Chirality. 2023 Dec;35(12):1019-1028. doi: 10.1002/chir.23616. Epub 2023 Aug 13.
Chiral high performance liquid chromatographic technique usually employs polysaccharide-based stationary phases in a normal phase mode. This frequently generates large waste of organic solvents. Using shorter columns of 50 mm length as well as a mobile phase with a high water percentage are common approaches for greening this analytical technique. In this context, a new chiral chromatographic technique was developed for simultaneous enantio-separation of phenylephrine HCl and guaifenesin racemates. Four 50 mm cellulose-based columns were experimented to separate the four enantiomers in a reversed phase mode. A face centered design was then employed to optimize the mobile phase acetonitrile% and flow rate on Lux Cellulose-1 (50 × 4.6 mm, 5 μm). The simultaneous resolution of the cited drugs enantiomers was achieved using acetonitrile-water (30:70, by volume), with a flow rate of 0.5 ml min . These optimized chromatographic conditions separate the enantiomers in 7 min running time, generating about 1.0 ml acetonitrile per run. The proposed method was favorably compared with other reported chiral ones in terms of waste volume generated and analysis time required.
手性高效液相色谱技术通常采用多糖类固定相在正相模式下进行。这通常会产生大量有机溶剂的浪费。采用 50mm 长度较短的色谱柱以及高水比例的流动相是使该分析技术绿色化的常见方法。在这种情况下，开发了一种新的手性色谱技术，用于同时对盐酸苯肾上腺素和愈创甘油醚外消旋体进行对映体分离。实验了四个 50mm 的纤维素基柱，以反相模式分离四个对映异构体。然后采用中心面设计优化 Lux Cellulose-1（50×4.6mm，5μm）的流动相乙腈%和流速。使用乙腈-水（30:70，体积比），流速为 0.5ml/min，实现了上述药物对映异构体的同时拆分。这些优化的色谱条件在 7min 的运行时间内分离对映异构体，每次运行产生约 1.0ml 的乙腈。就产生的废体量和所需的分析时间而言，与其他已报道的手性方法相比，该方法具有优势。
