Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Int J Lab Hematol. 2023 Dec;45(6):860-868. doi: 10.1111/ijlh.14145. Epub 2023 Aug 14.
To compare the morphological classification ability of peripheral-blood leukocytes of the automatic cell morphology analyzers MC-100i and DI-60.
(1) MC-100i and DI-60 were used to analyze leukocytes in 432 venous blood samples collected from three tertiary hospitals across China. The preclassification results were compared with the results reported by senior morphological experts (postclassification results) to evaluate the accuracy, sensitivity, specificity, and consistency of leukocyte preclassification for both instruments. (2) In 200 of the 432 blood samples, morphological experts conducted manual microscopic examination for various types of leukocytes. The correlation between the MC-100i and DI-60 leukocyte postclassification results and the expert microscopist results were analyzed.
(1) MC-100i preclassified leukocytes and nucleated red blood cells (RBCs). Compared with the postclassification results, the total leukocyte preclassification accuracy of MC-100i was 97.16%, while that of DI-60 was 87.24%. The sensitivity of MC-100i to abnormal cells (including blasts, promyelocytes, neutrophilic myelocytes, neutrophilic metamyelocytes, reactive lymphocytes, abnormal promyelocytes, plasma cells, abnormal lymphocytes and nucleated RBCs) was 90.24%, which was significantly higher than the 50.72% sensitivity of DI-60. (2) Comparing the postclassification results with manual microscopy, except for reactive lymphocytes and basophils, the MC-100i and DI-60 results had good correlations with various leukocyte types and nucleated RBCs (r > 0.85), and MC-100i was better than DI-60 in the recognition of basophils.
Both MC-100i and DI-60 have good detection ability for five normal types of leukocytes in peripheral blood. MC-100i has significantly better detection sensitivity for abnormal cells in peripheral blood than DI-60.
比较全自动血液细胞分析仪 MC-100i 和 DI-60 对末梢血白细胞形态学分类能力。
（1）采用 MC-100i 和 DI-60 对中国 3 家三甲医院采集的 432 份静脉血标本进行白细胞分析，将预分类结果与高级形态学专家报告的结果（后分类结果）进行比较，以评估两种仪器白细胞预分类的准确性、灵敏度、特异性和一致性。（2）在 432 份血样中的 200 份，形态学专家对各种白细胞进行了手工显微镜检查。分析 MC-100i 和 DI-60 白细胞后分类结果与专家显微镜检查结果的相关性。
（1）MC-100i 对白细胞和有核红细胞（RBC）进行预分类。与后分类结果比较，MC-100i 总白细胞预分类准确率为 97.16%，而 DI-60 为 87.24%。MC-100i 对异常细胞（包括原始细胞、早幼粒细胞、中性中幼粒细胞、中性晚幼粒细胞、反应性淋巴细胞、异常早幼粒细胞、浆细胞、异常淋巴细胞和有核 RBC）的灵敏度为 90.24%，明显高于 DI-60 的 50.72%。（2）后分类结果与手工显微镜比较，除反应性淋巴细胞和嗜碱性粒细胞外，MC-100i 和 DI-60 与各种白细胞类型和有核 RBC 均具有良好的相关性（r＞0.85），且 MC-100i 在识别嗜碱性粒细胞方面优于 DI-60。
MC-100i 和 DI-60 对末梢血中 5 种正常类型白细胞均具有良好的检测能力。MC-100i 对末梢血异常细胞的检测灵敏度明显优于 DI-60。
