Wang Yixin, Wang Zheng, Du Heshan, Chen Bin, Wang Guoyun, Wang Qian, Geng Sansheng, Zhang Xiaofen
Beijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing, China.
Front Plant Sci. 2023 Jul 27;14:1232755. doi: 10.3389/fpls.2023.1232755. eCollection 2023.
Pepper ( L) is one of the most important vegetables grown worldwide. Nevertheless, the key structural and regulatory genes involved in anthocyanin accumulation in pepper have not been well understood or fine mapped yet. In this study, F, F, BCP, and BCP pepper populations were analyzed and these populations were derived from a cross between line 14-Z4, which has yellow anthers and green stems, and line 14-Z5, which has purple anthers and stems. The results showed that the yellow anthers and green stems were determined by a single recessive locus called to as . While, using preliminary and fine mapping techniques, locus was located between markers aywSNP120 and aywSNP124, with physical distance of 0.2 Mb. The gene was identified as promising candidate for the locus, as it co-segregated with the yellow anthers and green stems phenotypes. encodes a homolog of the F3'5'H (flavonoid 3',5'-hydroxylase) anthocyanin synthesis structure gene. The missense mutation of possibly resulted in a loss-of-function. The expression analysis showed that was significantly expressed in the stems, leaves, anthers and petals in 14-Z5, and it's silencing caused the stems changing from purple to green. This study provides a theoretical basis for using yellow anthers and green stems in pepper breeding and helps to advance the understanding of anthocyanin synthesis.
辣椒（L）是全球种植的最重要蔬菜之一。然而，参与辣椒花青素积累的关键结构和调控基因尚未得到充分了解或精细定位。在本研究中，对F、F、BCP和BCP辣椒群体进行了分析，这些群体源自14-Z4品系（具有黄色花药和绿色茎）与14-Z5品系（具有紫色花药和茎）之间的杂交。结果表明，黄色花药和绿色茎由一个名为的单隐性位点决定。同时，利用初步和精细定位技术，将位点定位在标记aywSNP120和aywSNP124之间，物理距离为0.2 Mb。基因被确定为位点的有希望的候选基因，因为它与黄色花药和绿色茎表型共分离。编码F3'5'H（类黄酮3'，5'-羟化酶）花青素合成结构基因的同源物。的错义突变可能导致功能丧失。表达分析表明，在14-Z5的茎、叶、花药和花瓣中显著表达，其沉默导致茎从紫色变为绿色。本研究为辣椒育种中利用黄色花药和绿色茎提供了理论依据，并有助于推进对花青素合成的理解。
