Li Siyi, Guo Linjie, Gu Jinhong, Mu Guangqing, Tuo Yanfeng
School of Food Science and Technology Dalian Polytechnic University Dalian China.
Dalian Probiotics Function Research Key Laboratory Dalian Polytechnic University Dalian China.
Food Sci Nutr. 2023 Jul 19;11(8):4502-4515. doi: 10.1002/fsn3.3372. eCollection 2023 Aug.
Soybean paste was a traditional fermented product in northeast China, mainly fermented by molds, yeast, , and lactic acid bacteria. In this study, the safety and fermentation ability of lactic acid bacteria and yeast strains isolated from traditional soybean paste in northeast China were evaluated, and the dynamic changes of biogenic amines, aflatoxin, total acids, amino acid nitrogen, and volatile compounds were investigated during the fermentation of the traditional soybean paste. Among the tested strains, DPUL-J8 could decompose putrescine by 100%, and no biogenic amine was produced by DPUY-J8. DPUL-J8 and DPUY-J8 with strong biogenic amine degrading capacities were inoculated into the soybean paste. After 30 days of fermentation, the content of biogenic amines and aflatoxin in the fermented soybean paste declined by more than 60% and 50%, respectively. At the same time, compared with the control group without inoculation, the contents of total acid (1.29 ± 0.05 g/100 g), amino acid nitrogen (0.82 ± 0.01 g/100 g), and volatile compounds in soybean paste fermented by DPUL-J8 and DPUY-J8 were significantly increased, which had a good flavor. These results indicated that the use of DPUL-J8 and DPUY-J8 as starter cultures for soybean paste might be a good strategy to improve the safety and flavor of traditional Chinese soybean paste.
豆瓣酱是中国东北地区的传统发酵产品,主要由霉菌、酵母菌和乳酸菌发酵而成。本研究对从中国东北地区传统豆瓣酱中分离出的乳酸菌和酵母菌菌株的安全性及发酵能力进行了评估,并对传统豆瓣酱发酵过程中生物胺、黄曲霉毒素、总酸、氨基酸态氮和挥发性化合物的动态变化进行了研究。在所测试的菌株中,DPUL-J8能100%分解腐胺,DPUY-J8不产生生物胺。将具有较强生物胺降解能力的DPUL-J8和DPUY-J8接种到豆瓣酱中。发酵30天后,发酵豆瓣酱中生物胺和黄曲霉毒素的含量分别下降了60%以上和50%。同时,与未接种的对照组相比,由DPUL-J8和DPUY-J8发酵的豆瓣酱中总酸(1.29±0.05 g/100 g)、氨基酸态氮(0.82±0.01 g/100 g)和挥发性化合物的含量显著增加,风味良好。这些结果表明,使用DPUL-J8和DPUY-J8作为豆瓣酱的发酵剂可能是提高中国传统豆瓣酱安全性和风味的良好策略。