Danielsen H, Lindmo T, Reith A
Cytometry. 1986 Sep;7(5):475-80. doi: 10.1002/cyto.990070514.
A method is presented for determining ploidy distributions in mouse liver from image analysis with stereological estimations of nuclear size in tissue sections. Nuclear profile distributions obtained from profile measurements were subjected to a mathematical unfolding procedure in order to obtain the nuclear size distributions. Based on the assumption that nuclear size increases monotonically with nuclear DNA content, flow cytometric DNA analysis of suspensions of liver cell nuclei was used to calibrate the method, thus yielding the mean nuclear size of each ploidy class, i.e., diploid, tetraploid, and octaploid nuclei. After the size interval for each of the ploidy classes was determined, the method allowed determination of ploidy distributions in mouse liver by stereological image analysis alone. The method was established from combined stereological and flow cytometric measurements on liver tissue representing two different stages of liver regeneration after two-thirds partial hepatectomy, and it was tested against an independent set of data representing a marked increase in the portion of S-phase cells.
本文介绍了一种通过图像分析和组织切片中核大小的体视学估计来确定小鼠肝脏倍性分布的方法。从轮廓测量获得的核轮廓分布经过数学展开程序以获得核大小分布。基于核大小随核DNA含量单调增加的假设，利用肝细胞核悬浮液的流式细胞术DNA分析对该方法进行校准，从而得出每个倍性类别的平均核大小，即二倍体、四倍体和八倍体核。在确定每个倍性类别的大小区间后，该方法仅通过体视学图像分析就可以确定小鼠肝脏中的倍性分布。该方法是根据对代表三分之二部分肝切除术后肝脏再生两个不同阶段的肝脏组织进行的体视学和流式细胞术联合测量建立的，并针对一组代表S期细胞比例显著增加的独立数据进行了测试。
