Yun Xiaoyan, Kurz Marie J, Suri Rominder, McKenzie Erica R
Civil and Environmental Engineering Department, Temple University, Philadelphia, PA 19122, USA.
Academy of Natural Sciences of Drexel University, Philadelphia, PA 19103, USA.
MethodsX. 2023 Jul 20;11:102290. doi: 10.1016/j.mex.2023.102290. eCollection 2023 Dec.
QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample processing methods have previously been applied to a range of compounds and matrices. This study presents a modified QuEChERS sample processing method that was validated and employed for 24 per- and polyfluoroalkyl substances (PFAS) for various biological matrices. PFAS are a group of synthetic chemicals that have attracted substantial attention as some compounds are acknowledged to be persistent, toxic, and bioaccumulative. It is crucial to determine PFAS in diverse environmental matrices. Currently, limited sample processing methods for PFAS in biological matrices are available and the majority only focus on a few compounds such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA). Thus, there is a demand to develop a sample processing method which is effective for many commonly tested PFAS compounds in environmental biological samples. In this study, the detailed sample processing procedures and method performance are described. The highlights of this method are: •The extraction solvent and salts were adjusted for PFAS extraction from environmental biological matrices.•The modified QuEChERS method is effective for extraction and cleanup from a variety of matrices including algae, plants, invertebrates, amphibians, and fish.
QuEChERS（快速、简便、廉价、高效、耐用且安全）样品处理方法此前已应用于一系列化合物和基质。本研究提出了一种经过验证的改良QuEChERS样品处理方法，并将其用于各种生物基质中的24种全氟和多氟烷基物质（PFAS）的分析。PFAS是一类合成化学品，由于其中一些化合物被认为具有持久性、毒性和生物累积性，因此备受关注。在各种环境基质中测定PFAS至关重要。目前，生物基质中PFAS的样品处理方法有限，且大多数仅关注少数几种化合物，如全氟辛烷磺酸（PFOS）和全氟辛酸（PFOA）。因此，需要开发一种对环境生物样品中许多常见检测的PFAS化合物有效的样品处理方法。在本研究中，描述了详细的样品处理程序和方法性能。该方法的亮点包括：•调整了萃取溶剂和盐类，用于从环境生物基质中萃取PFAS。•改良的QuEChERS方法对于从包括藻类、植物、无脊椎动物、两栖动物和鱼类在内的各种基质中进行萃取和净化有效。
