Thabet Ahmed M, Alzuheir Ibrahim M, Laham Nahed A Al, Helal Belal Y Abu, Fayyad Adnan F, Jalboush Nasr H, Gharaibeh Mohammad H
Thabet Center for Veterinary Services diagnostic laboratory, Gaza Strip, Palestine.
Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Al-Azhar University, Gaza Strip, Palestine.
Vet World. 2023 Jun;16(6):1260-1265. doi: 10.14202/vetworld.2023.1260-1265. Epub 2023 Jun 8.
Colistin is used to treat avian pathogenic (APEC), a microorganism that affects turkey meat production in the Gaza Strip and worldwide. However, the recent emergence of plasmid-borne mobile colistin resistance () genes in pathogenic strains is a serious antimicrobial resistance (AMR) challenge for both human and animal health. In December 2018, colistin was banned as a veterinary antimicrobial in the Gaza Strip. This study aimed to detect and track the prevalence of colistin-resistant APEC isolated from turkey flocks in the Gaza Strip.
This study investigated 239 APEC isolates from turkey flocks in the Gaza Strip between October 2018 and December 2021 (at 6-month intervals). The colistin-resistant APEC strains were detected using the broth microdilution method. The gene was identified using a polymerase chain reaction.
The overall colistin resistance among the isolated APECs was 32.2% during the study period. The average resistance in the first interval was 37.5%, which significantly decreased to 9.3% in the last interval. Among the 77 phenotypically resistant isolates, 32.4% were positive for . The average abundance of -1 in the first interval was 66.6%, which decreased to 25% in the last interval.
To the best of our knowledge, this is the first study reporting the presence of the gene among the APEC isolates from turkeys in the Gaza Strip. Banned veterinary use of colistin significantly decreased the percentage of resistant APEC isolates from turkeys in Gaza Strip. Further studies are needed to investigate other colistin resistance genes and track the emergence of AMR.
黏菌素用于治疗禽致病性大肠杆菌（APEC），这种微生物影响加沙地带乃至全球的火鸡肉生产。然而，致病性大肠杆菌菌株中最近出现的质粒介导的可移动黏菌素耐药（mcr）基因，对人类和动物健康而言都是严峻的抗菌药物耐药性（AMR）挑战。2018年12月，黏菌素在加沙地带被禁止作为兽用抗菌药物使用。本研究旨在检测并追踪从加沙地带火鸡群中分离出的耐黏菌素APEC的流行情况。
本研究调查了2018年10月至2021年12月期间（每隔6个月）从加沙地带火鸡群中分离出的239株APEC分离株。采用肉汤微量稀释法检测耐黏菌素APEC菌株。使用聚合酶链反应鉴定mcr基因。
在研究期间，分离出的APEC中总体黏菌素耐药率为32.2%。第一个时间段的平均耐药率为37.5%，在最后一个时间段显著降至9.3%。在77株表型耐药分离株中，32.4%的mcr检测呈阳性。第一个时间段mcr-1的平均丰度为66.6%，在最后一个时间段降至25%。
据我们所知，这是第一项报道加沙地带火鸡APEC分离株中存在mcr基因的研究。禁止将黏菌素用于兽医领域显著降低了加沙地带火鸡中耐黏菌素APEC分离株的比例。需要进一步研究来调查其他黏菌素耐药基因并追踪AMR的出现情况。
