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从蜘蛛（斯科波利，1772年）毒腺转录组中鉴定新型抗菌肽

identification of novel antimicrobial peptides from the venom gland transcriptome of the spider (Scopoli, 1772).

作者信息

Shin Min Kyoung, Hwang In-Wook, Jang Bo-Young, Bu Kyung-Bin, Yoo Jung Sun, Sung Jung-Suk

机构信息

Department of Life Science, Dongguk University-Seoul, Goyang, Republic of Korea.

Species Diversity Research Division, National Institute of Biological Resources, Incheon, Republic of Korea.

出版信息

Front Microbiol. 2023 Jul 28;14:1249175. doi: 10.3389/fmicb.2023.1249175. eCollection 2023.

DOI:10.3389/fmicb.2023.1249175
PMID:37577428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10416796/
Abstract

As the emergence and prevalence of antibiotic-resistant strains have resulted in a global crisis, there is an urgent need for new antimicrobial agents. Antimicrobial peptides (AMPs) exhibit inhibitory activity against a wide spectrum of pathogens and can be utilized as an alternative to conventional antibiotics. In this study, two novel AMPs were identified from the venom transcriptome of the spider (Scopoli, 1772) using methods, and their antimicrobial activity was experimentally validated. Aranetoxin-Ab2a (AATX-Ab2a) and Aranetoxin-Ab3a (AATX-Ab3a) were identified by homology analysis and were predicted to have high levels of antimicrobial activity based on analysis. Both peptides were found to have antibacterial effect against Gram-positive and -negative strains, and, in particular, showed significant inhibitory activity against multidrug-resistant isolates. In addition, AATX-Ab2a and AATX-Ab3a inhibited animal and vegetable fungal strains, while showing low toxicity to normal human cells. The antimicrobial activity of the peptides was attributed to the increased permeability of microbial membranes. The study described the discovery of novel antibiotic candidates, AATX-Ab2a and AATX-Ab3a, using the spider venom gland transcriptome, and validated an -based method for identifying functional substances from biological resources.

摘要

由于抗生素耐药菌株的出现和流行已导致全球危机，因此迫切需要新型抗菌剂。抗菌肽（AMPs）对多种病原体具有抑制活性，可作为传统抗生素的替代品。在本研究中，使用相关方法从蜘蛛（斯科波利，1772年）的毒液转录组中鉴定出两种新型抗菌肽，并通过实验验证了它们的抗菌活性。通过同源性分析鉴定出蛛毒素-Ab2a（AATX-Ab2a）和蛛毒素-Ab3a（AATX-Ab3a），并基于相关分析预测它们具有高水平的抗菌活性。发现这两种肽对革兰氏阳性和阴性菌株均有抗菌作用，尤其对多重耐药分离株表现出显著的抑制活性。此外，AATX-Ab2a和AATX-Ab3a对动植物真菌菌株有抑制作用，而对正常人类细胞毒性较低。这些肽的抗菌活性归因于微生物膜通透性的增加。该研究描述了利用蜘蛛毒腺转录组发现新型抗生素候选物AATX-Ab2a和AATX-Ab3a，并验证了一种基于相关分析从生物资源中鉴定功能物质的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/528e791b3332/fmicb-14-1249175-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/14da046abc72/fmicb-14-1249175-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/6c038677f925/fmicb-14-1249175-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/5cf524d8ff2d/fmicb-14-1249175-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/a55db0f1f11e/fmicb-14-1249175-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/9f2ea8c8c33b/fmicb-14-1249175-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/29ef58565d07/fmicb-14-1249175-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/68960e80b32c/fmicb-14-1249175-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/528e791b3332/fmicb-14-1249175-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/14da046abc72/fmicb-14-1249175-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/6c038677f925/fmicb-14-1249175-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/5cf524d8ff2d/fmicb-14-1249175-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/a55db0f1f11e/fmicb-14-1249175-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/9f2ea8c8c33b/fmicb-14-1249175-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/29ef58565d07/fmicb-14-1249175-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/68960e80b32c/fmicb-14-1249175-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3154/10416796/528e791b3332/fmicb-14-1249175-g008.jpg

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Adv Drug Deliv Rev. 2022 Aug;187:114378. doi: 10.1016/j.addr.2022.114378. Epub 2022 Jun 4.
2
Biomaterial-based delivery of antimicrobial therapies for the treatment of bacterial infections.基于生物材料的抗菌疗法递送用于治疗细菌感染。
Nat Rev Mater. 2022 Jan;7(1):39-54. doi: 10.1038/s41578-021-00362-4. Epub 2021 Sep 15.
3
Antibiotic Resistance: One Health One World Outlook.抗生素耐药性：同一健康，同一世界的展望。
PA-Win2：基于计算机模拟发现具有双重抗菌和抗生物膜活性的新型肽。
Antibiotics (Basel). 2024 Nov 21;13(12):1113. doi: 10.3390/antibiotics13121113.
Front Cell Infect Microbiol. 2021 Nov 25;11:771510. doi: 10.3389/fcimb.2021.771510. eCollection 2021.
4
A Deep Learning Approach with Data Augmentation to Predict Novel Spider Neurotoxic Peptides.基于数据增强的深度学习方法预测新型蜘蛛神经毒素肽。
Int J Mol Sci. 2021 Nov 13;22(22):12291. doi: 10.3390/ijms222212291.
5
Antibiotic resistance in the environment.环境中的抗生素耐药性。
Nat Rev Microbiol. 2022 May;20(5):257-269. doi: 10.1038/s41579-021-00649-x. Epub 2021 Nov 4.
6
Artificial intelligence to deep learning: machine intelligence approach for drug discovery.人工智能到深度学习：药物发现的机器智能方法。
Mol Divers. 2021 Aug;25(3):1315-1360. doi: 10.1007/s11030-021-10217-3. Epub 2021 Apr 12.
7
Deep learning in next-generation sequencing.深度学习在下一代测序中的应用。
Drug Discov Today. 2021 Jan;26(1):173-180. doi: 10.1016/j.drudis.2020.10.002. Epub 2020 Oct 12.
8
Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider .蜘蛛新型肽毒素的抗菌和抗炎作用
Antibiotics (Basel). 2020 Jul 19;9(7):422. doi: 10.3390/antibiotics9070422.
9
Antimicrobials from Venomous Animals: An Overview.从有毒动物中提取的抗菌药物：概述。
Molecules. 2020 May 21;25(10):2402. doi: 10.3390/molecules25102402.
10
Threats Posed by the Fungal Kingdom to Humans, Wildlife, and Agriculture.真菌王国对人类、野生动物和农业构成的威胁。
mBio. 2020 May 5;11(3):e00449-20. doi: 10.1128/mBio.00449-20.