College of Resources and Environment, Qingdao Agricultural University Qingdao, PR China.
Qingdao Yizhong Testing Co, Qingdao, PR China.
Int J Phytoremediation. 2024 Feb;26(3):382-392. doi: 10.1080/15226514.2023.2243631. Epub 2023 Aug 14.
Based on the growth-promoting effect of plant growth promoting bacteria on plants and the mobilization of Cd by citric acid, an experiment was designed in which the combined treatment of Bacillus megaterium and citric acid promoted ryegrass to repair Cd-contaminated soil. This study aimed to evaluate the effects of different treatments on the antioxidant enzyme activity, photosynthesis intensity, Cd accumulation, and rhizosphere cadmium migration under cadmium contamination conditions. And the soil morphology and structure changes were studied by infrared spectroscopy FourierTransformInfrared(FT-IR) and scanning electron microscope Energy Dispersive Spectrometer(SEM-EDS) before and after different treatments. The results show that the combined treatment of Bacillus megaterium and citric acid significantly improved the oxidative stress defense and plant photosynthesis and increased of rye biomass. rye biomass 1.28 times higher than CK treatment. Joint treatment significantly increased the amount of shoot accumulation of Cd, 2.31 times higher than CK treatment, increased the migration and accumulation of cadmium. FTIR and SEM-EDS also showed that the organic constituents such as O-H, C-O and C-N in soils as a major mechanism for mobilization of the heavy metal Cd. Thus, the combined treatment of Bacillus megaterium and citric acid can promote plant growth, improve the damage to ryegrass caused by single organic acid addition, and improve the plant extraction efficiency, which is a feasible way to repair Cd-contaminated soil through activated extraction system.
基于植物促生菌对植物的促生作用和柠檬酸对 Cd 的活化作用，设计了一种利用巨大芽孢杆菌和柠檬酸联合处理修复 Cd 污染土壤的实验。本研究旨在评估不同处理方式对 Cd 污染条件下抗氧化酶活性、光合作用强度、Cd 积累和根际 Cd 迁移的影响，并通过傅里叶变换红外光谱（FT-IR）和扫描电子显微镜能谱（SEM-EDS）研究了不同处理前后土壤形态和结构的变化。结果表明，巨大芽孢杆菌和柠檬酸联合处理显著提高了氧化应激防御和植物光合作用，增加了黑麦草的生物量，比 CK 处理高 1.28 倍。联合处理显著增加了地上部 Cd 的积累量，比 CK 处理高 2.31 倍，增加了 Cd 的迁移和积累。FTIR 和 SEM-EDS 也表明，土壤中的 O-H、C-O 和 C-N 等有机成分是重金属 Cd 活化的主要机制。因此，巨大芽孢杆菌和柠檬酸的联合处理可以促进植物生长，改善单一有机酸添加对黑麦草造成的损害，提高植物的提取效率，这是通过激活提取系统修复 Cd 污染土壤的一种可行方法。
