School of Applied Engineering, Henan University of Science and Technology, Sanmenxia 472000, PR China.
School of Music, Henan University, Kaifeng 475001, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123249. doi: 10.1016/j.saa.2023.123249. Epub 2023 Aug 9.
Adenosine 5'-triphosphate (ATP) and guanosine 5'-triphosphate (GTP) are the most essential energy source in enormous biological processes. Various probes for ATP or GTP sensing, have been widely established, but the probe that could simultaneously monitor ATP and GTP is still rarely reported. Herein, we report a bipolar hemicyanine cationic probe for simultaneous sensing of ATP and GTP via a one-step monitoring process. This probe exhibited strong affinity to ATP and GTP through intramolecular electrostatic and π-π stacking interactions, which the binding constant on each step were determined as 6.15 × 10 M and 1.57 × 10 M for ATP, 3.19 × 10 M and 3.81 × 10 M for GTP. The sensitivity and specificity of this probe toward ATP or GTP over other twelve biological analogues (adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP), guanosine 5'-diphosphate (GDP), guanosine 5'-monophosphate (GMP), Etc.) have also been successfully demonstrated. Furthermore, due to the rapid response rate (within 10 s), we also proved that this probe could be employed as a monitor tool during the ATP or GTP-related enzymatic reaction process.
三磷酸腺苷 (ATP) 和三磷酸鸟苷 (GTP) 是许多生物过程中最基本的能量来源。已经广泛建立了各种用于检测 ATP 或 GTP 的探针，但能够同时监测 ATP 和 GTP 的探针仍很少见报道。在此，我们报告了一种双极半花菁阳离子探针，可通过一步监测过程同时检测 ATP 和 GTP。该探针通过分子内静电和π-π堆积相互作用与 ATP 和 GTP 具有很强的亲和力，每个步骤的结合常数分别确定为 6.15×10 M 和 1.57×10 M 用于 ATP，3.19×10 M 和 3.81×10 M 用于 GTP。该探针对 ATP 或 GTP 的灵敏度和特异性优于其他十二种生物类似物（二磷酸腺苷 (ADP)、一磷酸腺苷 (AMP)、二磷酸鸟苷 (GDP)、一磷酸鸟苷 (GMP) 等）也得到了成功证明。此外，由于响应速度快（<10 s），我们还证明该探针可在与 ATP 或 GTP 相关的酶反应过程中用作监测工具。
