Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
Bioresour Technol. 2023 Nov;387:129673. doi: 10.1016/j.biortech.2023.129673. Epub 2023 Aug 12.
Contamination of the environment with large amounts of residual oxytetracycline (OTC) and the corresponding resistance genes poses a potential threat to natural ecosystems and human health. In this study, an effective OTC-degrading strain, identified as Achromobacter denitrificans OTC-F, was isolated from activated sludge. In the degradation experiment, the degradation rates of OTC in the degradation systems with and without biochar addition were 95.01-100% and 73.72-99.66%, respectively. Biochar promotes the biodegradation of OTC, particularly under extreme environmental conditions. Toxicity evaluation experiments showed that biochar reduced biotoxicity and increased the proportion of living cells by 17.36%. Additionally, biochar increased the activity of antioxidant enzymes by 34.1-91.0%. Metabolomic analysis revealed that biochar promoted the secretion of antioxidant substances such as glutathione and tetrahydrofolate, which effectively reduced oxidative stress induced by OTC. This study revealed the mechanism at the molecular level and provided new strategies for the bioremediation of OTC in the environment.
大量残留土霉素（OTC）及其相应抗性基因对自然生态系统和人类健康构成潜在威胁。本研究从活性污泥中分离出一株有效降解 OTC 的菌株，鉴定为无色杆菌（Achromobacter denitrificans）OTC-F。在降解实验中，添加生物炭和不添加生物炭的降解体系中 OTC 的降解率分别为 95.01%-100%和 73.72%-99.66%。生物炭促进了 OTC 的生物降解，特别是在极端环境条件下。毒性评价实验表明，生物炭降低了生物毒性，使活细胞的比例增加了 17.36%。此外，生物炭还使抗氧化酶的活性提高了 34.1%-91.0%。代谢组学分析表明，生物炭促进了谷胱甘肽和四氢叶酸等抗氧化物质的分泌，有效减轻了 OTC 引起的氧化应激。本研究从分子水平揭示了其作用机制，为环境中土霉素的生物修复提供了新策略。
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