Oda Y, Minami K
Eur J Biochem. 1986 Sep 1;159(2):239-45. doi: 10.1111/j.1432-1033.1986.tb09859.x.
A lectin, which agglutinated specifically the yeast cells of the Saccharomyces genus, was isolated from tulip bulbs (Tulipa gesneriana) using affinity chromatography on mannan-Sepharose 4B. Its relative molecular mass was determined by gel filtration to be approximately 67,000. On polyacrylamide gel electrophoresis in sodium dodecyl sulfate, a relative molecular mass of 17,000 was obtained, suggesting that the lectin is a tetramer. Binding studies performed with iodinated lectin indicated that Saccharomyces cerevisiae cells contained approximately 5.7 X 10(6) binding sites per cell, whereas little binding was observed with yeasts other than the Saccharomyces genus, bacteria and animal erythrocytes. D-Mannose, D-mannose 6-phosphate, L-fucose and L-fucosylamine were potent inhibitors of the lectin binding to S. cerevisiae cells, while, D-glucose, D-galactose and D-mannosamine were inactive, indicating that hydroxyl group at C-2 of D-mannose was essential for the lectin binding. Furthermore, inhibition experiments, using various manno-oligosaccharides, suggested that the lectin recognized (1----6)-linked manno-oligosaccharide units larger than mannobiose.
利用甘露聚糖-琼脂糖4B亲和层析法从郁金香球茎（郁金香）中分离出一种能特异性凝集酿酒酵母属酵母细胞的凝集素。通过凝胶过滤法测定其相对分子质量约为67,000。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中，得到的相对分子质量为17,000，表明该凝集素是一种四聚体。用碘化凝集素进行的结合研究表明，酿酒酵母细胞每个细胞含有约5.7×10⁶个结合位点，而除酿酒酵母属外的其他酵母、细菌和动物红细胞几乎没有观察到结合。D-甘露糖、D-甘露糖6-磷酸、L-岩藻糖和L-岩藻糖胺是凝集素与酿酒酵母细胞结合的有效抑制剂，而D-葡萄糖、D-半乳糖和D-甘露糖胺则无活性，表明D-甘露糖C-2位的羟基对于凝集素结合至关重要。此外，使用各种甘露寡糖的抑制实验表明，该凝集素识别大于甘露二糖的（1→6）连接的甘露寡糖单元。
