Van Kuik J A, Van Halbeek H, Kamerling J P, Vliegenthart J F
Eur J Biochem. 1986 Sep 1;159(2):297-301. doi: 10.1111/j.1432-1033.1986.tb09867.x.
The N-glycosidic carbohydrate chains of Panulirus interruptus hemocyanin, consisting of only mannose and N-acetylglucosamine residues, were liberated by hydrazinolysis of a pronase digest of the purified glycoprotein. The carbohydrate chains were fractionated by high-voltage paper electrophoresis, yielding mainly neutral oligosaccharide-alditols. These were further separated by high-performance liquid chromatography and characterized by 500-MHz 1H-NMR spectroscopy as (formula; see text).
