Tucker R W, Snowdowne K W, Borle A B
Eur J Cell Biol. 1986 Aug;41(2):347-51.
In order to investigate how growth factors stimulate DNA synthesis, we studied the relationship between early increases in the intracellular concentration of free calcium (Cai) and early mitogenic events (competence) in quiescent BALB/c 3T3 cells. Cai was measured by aequorin luminescence, and DNA synthesis was quantitated by autoradiography. While both serum (2%) and platelet-derived growth factor (2 U/ml) stimulated large increases in Cai without inducing competence, fibroblast growth factor (100-400 ng/ml) produced a large number of competent cells without causing a detectable increase in Cai. Thus, there was no correlation between the magnitude of Cai increase and subsequent competence for DNA synthesis.
为了研究生长因子如何刺激DNA合成，我们研究了静止的BALB/c 3T3细胞中游离钙（Cai）细胞内浓度的早期升高与早期促有丝分裂事件（感受态）之间的关系。通过水母发光蛋白发光测量Cai，通过放射自显影法定量DNA合成。虽然血清（2%）和血小板衍生生长因子（2 U/ml）均刺激Cai大幅升高但未诱导感受态，而成纤维细胞生长因子（100 - 400 ng/ml）产生大量感受态细胞却未导致Cai出现可检测到的升高。因此，Cai升高的幅度与随后的DNA合成感受态之间没有相关性。
