Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research, Raebareli, Transit Campus Lucknow, UP, India.
Department of Pharmacology & Toxicology/Regulatory Toxicology, National Institute of Pharmaceutical Education and Research, Raebareli, Transit Campus Lucknow, UP, India.
Analyst. 2023 Sep 11;148(18):4513-4524. doi: 10.1039/d3an00870c.
Copper is one of the most important transition metals in our body for various physiological functions. An imbalance in the homeostasis of copper in our body can lead to various neurological disorders such as Alzheimer's disease, Parkinson's, and Menkes disease. As a result, there is a need for the detection of excess copper present in the environment and the human system. In this work, we have designed a quinoline-based fluorescent/colorimetric probe (QHS) for rapid and selective detection of copper ions quenching of fluorescence/color change from yellow to peach which is visible to the naked eye. The probe displayed high selectivity towards copper(II), , Cu(II) in the presence of different metal analytes in water samples. The sensing mechanism of the probe was confirmed by NMR, HRMS, IR spectroscopy, and SEM. The detection limit of Cu(II) ions was found to be 0.493 μM which is lower than the tolerable limit of copper (20 μM) in drinking water as per the EPA. The probe was also utilized for the visualization of Cu(II) in cell lines. The probe was also demonstrated for its application in real-time detection of aqueous samples using portable paper strips.
铜是我们体内各种生理功能的最重要的过渡金属之一。体内铜的动态平衡失衡会导致各种神经紊乱，如阿尔茨海默病、帕金森病和 Menkes 病。因此，需要检测环境和人体系统中存在的过量铜。在这项工作中，我们设计了一种基于喹啉的荧光/比色探针（QHS），用于快速选择性检测铜离子，荧光/颜色从黄色变为桃红色，肉眼可见。该探针在水样中存在不同金属分析物的情况下，对铜（II）、Cu(II)具有高选择性。通过 NMR、HRMS、IR 光谱和 SEM 证实了探针的传感机制。发现 Cu(II)离子的检测限为 0.493 μM，低于 EPA 规定的饮用水中铜的耐受极限（20 μM）。该探针还用于细胞系中 Cu(II)的可视化。该探针还展示了其在使用便携式纸条实时检测水样中的应用。
