植入前牛胚胎中DKK1的KREMEN受体的存在。
Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.
作者信息
Amaral Thiago Fernandes, Xiao Yao, Jeensuk Surawich, Maia Tatiane Silva, Cuellar Camila J, Gingerich Chloe A, Scheffler Tracy L, Hansen Peter J
机构信息
T Amaral, Animal Sciences, University of Florida, Gainesville, United States.
Y Xiao, Key Laboratory of Livestock and Poultry Multi-omics of MARA, Shandong Academy of Agricultural Sciences, Jinan, China.
出版信息
Reprod Fertil. 2023 Aug 1;4(4). doi: 10.1530/RAF-23-0021.
The WNT inhibitory protein DKK1 has been shown to regulate development of the preimplantation embryo to the blastocyst stage. In cattle, DKK1 increases the number of trophectoderm cells that are the precursor of the placenta. DKK1 can affect cells by blocking WNT signaling through its receptors KREMEN1 and KREMEN2. Here it was shown that the mRNA for KREMEN1 and KREMEN2 decline as the embryo advances in development. Nonetheless, immunoreactive KREMEN1 was identified in blastocysts using Western blotting. DKK1 also decreased amount of immunoreactive CTNNB1 in blastocysts, as would be expected if DKK1 was signaling through a KREMEN-mediated pathway. Thus, it is likely that KREMEN1 functions as a receptor for DKK1 in the preimplantation bovine embryo.
WNT抑制蛋白DKK1已被证明可调节植入前胚胎发育至囊胚阶段。在牛中，DKK1可增加作为胎盘前体的滋养外胚层细胞的数量。DKK1可通过其受体KREMEN1和KREMEN2阻断WNT信号传导来影响细胞。本文表明，随着胚胎发育推进，KREMEN1和KREMEN2的mRNA水平下降。尽管如此，通过蛋白质免疫印迹法在囊胚中鉴定出了具有免疫反应性的KREMEN1。如DKK1通过KREMEN介导的途径进行信号传导所预期的那样，DKK1还降低了囊胚中具有免疫反应性的CTNNB1的量。因此，KREMEN1很可能在植入前牛胚胎中作为DKK1的受体发挥作用。
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