Xue Shan, Wang Shuya, Liu Li, Zhong Qiaofang, Cheng Zaiquan, Xiao Suqin
School of Agriculture, Yunnan University, Kunming 650504, Yunnan, China.
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Jul 25;39(7):2566-2578. doi: 10.13345/j.cjb.220879.
Gene editing technology is a genetic operation technology that can modify the DNA sequence at the genomic level. The precision gene editing technology based on CRISPR/Cas9 system is a gene editing technology that is easy to operate and widely used. Unlike the traditional CRISPR/Cas9 system, the precision gene editing technology can perform site-directed mutation of genes without DNA template. This review summarizes the recent development of precision gene editing technology based on CRISPR/Cas9, and prospects the challenges and opportunities of this technology.
基因编辑技术是一种能够在基因组水平上修饰DNA序列的遗传操作技术。基于CRISPR/Cas9系统的精准基因编辑技术是一种易于操作且应用广泛的基因编辑技术。与传统的CRISPR/Cas9系统不同，精准基因编辑技术无需DNA模板即可对基因进行定点突变。本文综述了基于CRISPR/Cas9的精准基因编辑技术的最新进展，并展望了该技术面临的挑战与机遇。
