[猪流行性腹泻mRNA疫苗的制备及免疫原性评价]
[Preparation and immunogenicity evaluation of mRNA vaccine against porcine epidemic diarrhea].
作者信息
Yang Limin, Wang Junhong, Xu Mingguo, Wang Hu, Zhang Xiaojuan, Liu Wenjun, Chen Chuangfu
机构信息
Key Laboratory of Zoonoses, College of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, China.
Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2023 Jul 25;39(7):2624-2633. doi: 10.13345/j.cjb.220853.
Porcine epidemic diarrhea (PED) is a highly contagious disease that causes high mortality in suckling piglets. Although several licensed inactivated and live attenuated vaccines were widely used, the infection rate remains high due to unsatisfactory protective efficacy. In this study, mRNA vaccine candidates against PED were prepared, and their immunogenicity was evaluated in mice and pregnant sows. The mRNA PED vaccine based on heterodimer of viral receptor binding region (RBD) showed good immunogenicity. It elicited robust humoral and cellular immune responses in mice, and the neutralizing antibody titer reached 1:300 after a single vaccination. Furthermore, it induced neutralizing antibody level similar to that of the inactivated vaccine in pregnant sows. This study developed a new design of PED vaccine based on the mRNA-RBD strategy and demonstrated the potential for clinical application.
猪流行性腹泻（PED）是一种高度传染性疾病，可导致哺乳仔猪的高死亡率。尽管几种已获许可的灭活疫苗和减毒活疫苗被广泛使用，但由于保护效果不理想，感染率仍然很高。在本研究中，制备了针对PED的mRNA候选疫苗，并在小鼠和怀孕母猪中评估了它们的免疫原性。基于病毒受体结合区（RBD）异二聚体的mRNA PED疫苗显示出良好的免疫原性。它在小鼠中引发了强烈的体液免疫和细胞免疫反应，单次接种后中和抗体滴度达到1:300。此外，它在怀孕母猪中诱导的中和抗体水平与灭活疫苗相似。本研究基于mRNA-RBD策略开发了一种新型的PED疫苗设计，并证明了其临床应用潜力。
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