Universidade Federal de Lavras (UFLA), Departamento de Agricultura, Laboratório de Cultura de Tecidos Vegetais, Caixa Postal 3037, Campus Universitário, 37200-900 Lavras, MG, Brazil.
Texas Woman's University, Laboratory of Biology, Denton, TX 76204, USA.
An Acad Bras Cienc. 2023 Aug 11;95(2):e20200021. doi: 10.1590/0001-3765202320200021. eCollection 2023.
Diatomaceous earth is an organic naturally occurring material rich in silicon. This silicon source can be used in organic agriculture, it also has a great potential of use in the acclimatization of crops. However, there are no reports of the effects of diatomaceous earth supplementation on the micropropagation of sweet potato. Thus, the objective of this study was to evaluate the effects of different concentrations of diatomaceous earth applied in vitro on the growth, physiology and anatomy of sweet potato cv. 'Brazlândia Branca' after acclimatization. Four concentrations of diatomaceous earth. After 30 days of in vitro growth, the plants were transferred to a greenhouse for acclimatization. After 45 days, leaf number, shoot and root length, fresh and dry shoot and root mass, gas exchange, chlorophyll content, root and leaf anatomy. The experimental design was completely randomized. The supplementation of diatomaceous earth in the in vitro cultivation had beneficial effects, increasing the accumulation of mass, improving the photosynthetic apparatus and promoting favorable anatomical characteristics during the acclimatization of the sweet potato plants. In addition, the use of diatomaceous earth achieved adequate seedling development, with higher seedling quality and resistance to biotic and abiotic effects than attained with control treatment.
硅藻土是一种有机的天然硅元素富矿材料。这种硅源可以用于有机农业，也有很大的潜力用于作物的驯化。然而，目前还没有关于补充硅藻土对甘薯微繁殖影响的报道。因此，本研究的目的是评估不同浓度的硅藻土在体外对驯化后甘薯品种'Brazlândia Branca'生长、生理和解剖结构的影响。使用了四种浓度的硅藻土。在体外生长 30 天后，将植物转移到温室中进行驯化。45 天后，测量叶片数量、茎和根的长度、茎和根的鲜重和干重、气体交换、叶绿素含量、根和叶的解剖结构。实验设计采用完全随机化。在体外培养中补充硅藻土具有有益的效果，增加了质量的积累，改善了光合作用器，并在甘薯植物的驯化过程中促进了有利的解剖学特征。此外，使用硅藻土实现了充足的幼苗发育，与对照处理相比，具有更高的幼苗质量和对生物和非生物影响的抗性。
