Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Universidade Federal de Goiás, Instituto de Ciências Biológicas, Avenida Esperança, s/n, Campus Samambaia, 74690-900 Goiânia, GO, Brazil.
Universidade Federal de Goiás, Instituto de Ciências Biológicas, Avenida Esperança, s/n, Campus Samambaia, 74690-900 Goiânia, GO, Brazil.
An Acad Bras Cienc. 2023 Aug 14;95(suppl 1):e20201578. doi: 10.1590/0001-3765202320201578. eCollection 2023.
The diversity of eukaryotic and prokaryotic communities has been assessed by morphological and genetic approaches, which are used to characterize the microbiota in different environments. Here, planktonic prokaryotic and eukaryotic communities of the Araguaia River, located in the Central region of Brazil, were analyzed based on metabarcoding analysis of rRNA genes to evaluate the diversity of these groups in tropical floodplain lakes. Also, we tested their spatial concordance throughout the Araguaia river. Water samples were collected from 8 floodplain lakes in Araguaia River. The 16S and 18S rRNA genes were amplified and sequenced using Illumina MiSeq. For eukaryotes, 34,242 merged reads were obtained and 225 distinct OTUs were delineated, of which 106 OTUs were taxonomically classified. For prokaryotes, 26,426 sequences were obtained and 351 OTUs were detected. Of them, 231 were classified in at least one taxonomic category. The most representative eukaryotes belonged to Ciliophora, Chlorophyta and Charophyta. The prokaryotic phylum with the most OTUs classified were Proteobacteria, Actinobacteria and Bacteroidetes. The lakes did not show spatial concordance when comparing the similarity between their microbiota. The knowledge of freshwater biodiversity using DNA sequencing for important rivers, such as Araguaia River, can improve microbiota inventories of tropical biodiversity hotspots.
真核生物和原核生物群落的多样性已通过形态学和遗传学方法进行了评估，这些方法用于表征不同环境中的微生物群。在这里，根据 rRNA 基因的代谢组学分析，对巴西中部 Araguaia 河的浮游原核生物和真核生物群落进行了分析，以评估这些群体在热带洪泛区湖泊中的多样性。此外，我们还测试了它们在整个 Araguaia 河上的空间一致性。从 Araguaia 河的 8 个洪泛区湖泊中采集水样。使用 Illumina MiSeq 扩增和测序 16S 和 18S rRNA 基因。对于真核生物，获得了 34,242 个合并的读数，并划分了 225 个独特的 OTUs，其中 106 OTUs进行了分类。对于原核生物，获得了 26,426 个序列，并检测到 351 个 OTUs。其中，231 个在至少一个分类类别中进行了分类。最具代表性的真核生物属于纤毛门、绿藻门和裸藻门。分类最多的 OTUs 的原核生物门是变形菌门、放线菌门和拟杆菌门。当比较微生物群之间的相似性时，这些湖泊没有表现出空间一致性。使用 DNA 测序对 Araguaia 河等重要河流进行淡水生物多样性的研究，可以提高热带生物多样性热点地区的微生物组清单。
