小青龙汤通过 IL-33/ST2 和 JAK/STAT 通路抑制 ILC2s 改善 OVA 诱导的变应性鼻炎。
Xiao-qing-long-tang ameliorates OVA-induced allergic rhinitis by inhibiting ILC2s through the IL-33/ST2 and JAK/STAT pathways.
机构信息
The First Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China; Department of Otorhinolaryngology, the Second People's Hospital of Foshan, Affiliated Foshan Hospital of Southern Medical University, Foshan, Guangdong Province, 528099, China.
The First Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
出版信息
Phytomedicine. 2023 Oct;119:155012. doi: 10.1016/j.phymed.2023.155012. Epub 2023 Aug 6.
BACKGROUND
Allergic rhinitis (AR) is a chronic inflammatory disease of the nasal mucosa that is mediated by immunoglobulin E (IgE). Xiao-qing-long-tang (XQLT) is a traditional Chinese medicine compound that is widely used to treat respiratory diseases such as AR. However, the underlying mechanism of the effect of XQLT on AR remains unclear.
PURPOSE
To elucidate the effect of XQLT on ovalbumin (OVA)-induced AR and the mechanisms of action.
METHODS
The therapeutic efficacy of XQLT was evaluated in a well-established OVA-induced AR mouse model. Nasal symptoms were analyzed, type 2 cytokines and OVA-sIgE levels were measured, nasal mucosa tissues were collected for histological analysis, and the changes of Group 2 innate lymphoid cells (ILC2s) and the IL-33/ST2 and JAK/STAT signaling pathways in the nasal mucosa were observed.
RESULTS
XQLT significantly alleviated the nasal symptoms and histological damage to the nasal mucosa in AR mice, and reduced the levels of type 2 cytokines and OVA-sIgE. In addition, after XQLT treatment, the numbers of ILC2s in the nasal mucosa of AR mice were reduced, and the mRNA levels of the transcription factors GATA3 and ROR-α were decreased. Moreover, IL-33/ST2 signaling pathway was inhibited. The costimulatory cytokine associated JAK/STAT signaling pathway was also inhibited in ILC2s.
CONCLUSION
Our study demonstrated that XQLT regulated ILC2s through the IL-33/ST2 and JAK/STAT pathways to ameliorate type 2 inflammation in OVA-induced AR. These findings suggest that XQLT might be used to treat AR.
背景
变应性鼻炎（AR）是一种由免疫球蛋白 E（IgE）介导的鼻黏膜慢性炎症性疾病。小青龙汤（XQLT）是一种中药复方，广泛用于治疗 AR 等呼吸道疾病。然而，XQLT 治疗 AR 的作用机制尚不清楚。
目的
阐明 XQLT 对卵清蛋白（OVA）诱导的 AR 的作用及其作用机制。
方法
在建立良好的 OVA 诱导的 AR 小鼠模型中评价 XQLT 的治疗效果。分析鼻症状，测量 2 型细胞因子和 OVA-sIgE 水平，收集鼻黏膜组织进行组织学分析，观察 2 型固有淋巴细胞（ILC2）和 IL-33/ST2 及 JAK/STAT 信号通路的变化在鼻黏膜中。
结果
XQLT 显著缓解 AR 小鼠的鼻症状和鼻黏膜组织学损伤，降低 2 型细胞因子和 OVA-sIgE 水平。此外，XQLT 治疗后，AR 小鼠鼻黏膜中 ILC2 的数量减少，转录因子 GATA3 和 ROR-α 的 mRNA 水平降低。此外，IL-33/ST2 信号通路被抑制。ILC2 中的共刺激细胞因子相关 JAK/STAT 信号通路也被抑制。
结论
本研究表明，XQLT 通过 IL-33/ST2 和 JAK/STAT 通路调节 ILC2，改善 OVA 诱导的 AR 中的 2 型炎症。这些发现表明 XQLT 可能用于治疗 AR。
Zotero 插件