Department of Anesthesiology and Surgical Intensive Care Unit, Xinhua Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China; State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Pathology Department, Yantai Fushan People's Hospital, Yantai, China.
Biosens Bioelectron. 2023 Oct 15;238:115587. doi: 10.1016/j.bios.2023.115587. Epub 2023 Aug 9.
Methylation of the promoter region of cancer related genes plays a crucial role in the occurrence and development of cancer, and the degree of methylation has great potential for the early cancer diagnosis. At present, the technology used to quantify DNA methylation is mainly based on the DNA sequencing which are time-consuming and high-cost in the relating application. We have developed an ultrasensitive method of methylation specific enzyme-linked oligonucleotide assays (MS-ELONA) to detect and quantify the level of DNA methylation. We could detect as little as 2 pg of methylated DNA in the 100000-fold excess of unmethylated genes, and discriminate prostate cancer from benign prostatic hyperplasia (BPH) and control with serum samples. We also demonstrate the reversibility of DNA methylation modification by treatment with demethylation drugs. With 16-channel electrochemical work station, our research reveals a simple and inexpensive method to quantify the methylation level of specially appointed genes, and have the potential to be applied in the clinical research.
癌症相关基因启动子区域的甲基化在癌症的发生和发展中起着至关重要的作用，甲基化的程度对癌症的早期诊断具有很大的潜力。目前，用于定量 DNA 甲基化的技术主要基于 DNA 测序，在相关应用中既耗时又昂贵。我们已经开发了一种超灵敏的甲基化特异性酶联寡核苷酸检测（MS-ELONA）方法来检测和定量 DNA 甲基化水平。我们可以在 100000 倍过量的未甲基化基因中检测到少至 2pg 的甲基化 DNA，并使用血清样本区分前列腺癌与良性前列腺增生（BPH）和对照。我们还证明了 DNA 甲基化修饰的可逆性，通过使用去甲基化药物进行处理。通过使用 16 通道电化学工作站，我们的研究揭示了一种简单且廉价的定量指定基因甲基化水平的方法，并且有可能应用于临床研究。
