Constantine The Philosopher University in Nitra, Tr. A. Hlinku 1, 949 74 Nitra, Slovakia.
Constantine The Philosopher University in Nitra, Tr. A. Hlinku 1, 949 74 Nitra, Slovakia.
Reprod Biol. 2023 Sep;23(3):100795. doi: 10.1016/j.repbio.2023.100795. Epub 2023 Aug 14.
The aim of the present in-vitro experiments was to examine the direct influence of ghrelin and obestatin on viability, proliferation and progesterone release by human ovarian granulosa cells and their response to FSH administration. Human granulosa cells were cultured in presence of ghrelin or obestatin (both at 0, 1, 10 or 100 ng/ml) alone or in the presence of FSH (10 ng/ml). Cell viability, accumulation of proliferation markers PCNA and cyclin B1 and release of progesterone were analyzed by Trypan blue extrusion test, quantitative immunocytochemistry and ELISA. Ghrelin, obestatin and FSH up-regulated all the measured ovarian cell parameters. Moreover, both ghrelin and obestatin promoted all the stimulatory effects of FSH. The obtained results demonstrate the direct stimulatory action of ghrelin, obestatin and FSH on basic ovarian cell functions, as well as the ability of metabolic hormones to improve FSH action on human ovarian cells.
本体外实验旨在研究生长激素释放肽（ghrelin）和肥胖抑制素（obestatin）对人卵巢颗粒细胞活力、增殖和孕激素分泌的直接影响及其对促卵泡激素（FSH）给药的反应。将人卵巢颗粒细胞在生长激素释放肽或肥胖抑制素（均为 0、1、10 或 100ng/ml）单独或存在促卵泡激素（10ng/ml）的情况下培养。通过台盼蓝排斥试验、定量免疫细胞化学和 ELISA 分析细胞活力、增殖标志物 PCNA 和细胞周期蛋白 B1 的积累以及孕激素的释放。生长激素释放肽、肥胖抑制素和 FSH 均上调了所有测量的卵巢细胞参数。此外，生长激素释放肽和肥胖抑制素均促进了 FSH 的所有刺激作用。研究结果表明，生长激素释放肽、肥胖抑制素和 FSH 对基本的卵巢细胞功能具有直接的刺激作用，代谢激素能够增强 FSH 对人卵巢细胞的作用。
