Puchkova T V, Putvinsky A V, Parnev O M
Gen Physiol Biophys. 1986 Jun;5(3):231-42.
The electrical breakdown potential of the planar lipid membranes has been shown to decrease following UV-induced lipid peroxidation, action of phospholipase A2, adsorption of protamine sulphate and expansion of the membrane by hydrostatic pressure. Membrane potential generated upon the addition of potassium acetate (or ammonium sulphate) and protonophore CCCP to liposomes, when large enough, was also able to break membranes; this was suggested by liposome swelling and a rapid decrease in suspension turbidity. UV-irradiation decreased liposomal membrane breakdown potential, while cholesterol increased it. Detergents and water-soluble products of lipid peroxidation decreased the breakdown potential. The possible role of the membrane electrical breakdown phenomenon in cell pathology is discussed.
平面脂质膜的电击穿电位已被证明会在紫外线诱导的脂质过氧化、磷脂酶A2的作用、硫酸鱼精蛋白的吸附以及静水压使膜膨胀后降低。当向脂质体中添加醋酸钾（或硫酸铵）和质子载体CCCP时产生的膜电位，若足够大，也能够使膜破裂；脂质体肿胀和悬浮液浊度迅速降低表明了这一点。紫外线照射降低了脂质体膜的击穿电位，而胆固醇则提高了它。洗涤剂和脂质过氧化的水溶性产物降低了击穿电位。本文讨论了膜电击穿现象在细胞病理学中的可能作用。
