Guangxi Key Laboratory of Animal Breeding and Disease Control, Guangxi University, Nanning, 530005, China.
Guangxi Key Laboratory of Animal Breeding and Disease Control, Guangxi University, Nanning, 530005, China.
Toxicon. 2023 Sep;233:107256. doi: 10.1016/j.toxicon.2023.107256. Epub 2023 Aug 15.
Oocyte aging directly affects the subsequent embryonic development. Epifriedelanol is the active ingredient of Aster tataricus L.F. extract, and it possesses potential anti-cancer, anti-inflammatory and antioxidant properties. In addition, epifriedelanol can slow the aging of human skin fibroblasts. To explore the effect of epifriedelanol on the aging of porcine oocytes matured in vitro, the aging model was first established, epifriedelanol was added to in vitro maturation (IVM) medium to investigate its anti-aging effects by observing oocyte maturation and embryonic development potential, and analyzing aging-related gene expression, reactive oxygen species and mitochondrial membrane potential levels. It was found that typical aging of porcine oocytes appeared from 66 h during in vitro maturation. Compared with the 44 h group, a larger perivitelline space, increased abnormality of microtubulin formation, and significantly lower blastocyst rate were observed in the 66 h and 72 h groups. Compared with the 0 μg/mL group, the first polar body extrusion, cleavage and blastocyst rates were significantly improved (P < 0.05) in 10 μg/mL group. The expression of oocyte developmental potential-related, SIRT family-related, antioxidant and anti-apoptotic-related genes was significantly up-regulated (P < 0.05), p53 and pro-apoptotic genes were significantly down-regulated (P < 0.05). In addition, the reactive oxygen species level was significantly decreased (P < 0.01), the mitochondrial membrane potential was significantly elevated (P < 0.01) in 10 μg/mL group. In conclusion, epifriedelanol delays the aging of porcine oocytes cultured in vitro by up-regulating SIRT family gene expression, enhancing the antioxidant and anti-apoptotic capacity of oocytes.
卵母细胞衰老直接影响随后的胚胎发育。表friedelan-3β-醇是苍术提取物的活性成分，具有潜在的抗癌、抗炎和抗氧化特性。此外，表friedelan-3β-醇可以减缓人皮肤成纤维细胞的衰老。为了探讨表friedelan-3β-醇对体外成熟猪卵母细胞衰老的影响，首先建立衰老模型，在体外成熟（IVM）培养基中加入表friedelan-3β-醇，通过观察卵母细胞成熟和胚胎发育潜能，分析衰老相关基因表达、活性氧和线粒体膜电位水平，研究其抗老化作用。结果表明，猪卵母细胞在体外成熟 66 h 时出现典型衰老。与 44 h 组相比，66 h 和 72 h 组卵母细胞的卵周隙增大，微管形成异常增加，囊胚率显著降低。与 0 μg/ml 组相比，10 μg/ml 组第一极体排出、卵裂和囊胚率均显著提高（P < 0.05）。卵母细胞发育潜能相关、SIRT 家族相关、抗氧化和抗凋亡相关基因表达显著上调（P < 0.05），p53 和促凋亡基因表达显著下调（P < 0.05）。此外，10 μg/ml 组活性氧水平显著降低（P < 0.01），线粒体膜电位显著升高（P < 0.01）。综上所述，表friedelan-3β-醇通过上调 SIRT 家族基因表达，增强卵母细胞的抗氧化和抗凋亡能力，延缓体外培养的猪卵母细胞衰老。
