Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, China.
Department of Dentistry, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, China.
J Periodontal Res. 2023 Dec;58(6):1201-1211. doi: 10.1111/jre.13176. Epub 2023 Aug 16.
To evaluate the effect of hyperlipidemia on the healing of bone defects.
Apolipoprotein E (ApoE)-deficient mice and wild-type (WT) C57BL/6J mice were fed with an atherogenic high-fat diet (HFD) or a standard chow diet (as control) for 6 weeks. Blood samples were collected to evaluate serum lipid levels. Closed bone defects and open tooth extraction wounds were then created in the mandibles of these animals. One or two weeks after surgery, animals were euthanized. Micro-CT analysis and histomorphometric analysis were conducted to evaluate the healing of bone defects and the alveolar ridge resorption.
Bone regeneration of closed bone defects was considerably delayed in the hyperlipidemic Apoe-/- mice and WT mice. No obvious difference was detected in the new bone formation of the tooth extraction wounds. The HFD-fed mice showed more prominent reduction in the lingual alveolar ridge height of the tooth extraction wounds when compared with the control group.
Hyperlipidemia results in delayed bone regeneration in large closed bone defects. Although tooth extraction wounds are small and normally regenerated in a hyperlipidemic microenvironment, the prominent reduction in the alveolar ridge height is also a challenge for future restoration of the dentition.
评估高血脂对骨缺损愈合的影响。
载脂蛋白 E（ApoE）缺陷型小鼠和野生型（WT）C57BL/6J 小鼠分别用动脉粥样硬化性高脂饮食（HFD）或标准饲料（作为对照）喂养 6 周。采集血样以评估血清脂质水平。然后在这些动物的下颌骨中创建闭合性骨缺损和开放性拔牙创口。手术后 1 或 2 周，处死动物。进行 micro-CT 分析和组织形态计量学分析，以评估骨缺损的愈合和牙槽嵴吸收情况。
在高脂血症 Apoe-/-小鼠和 WT 小鼠中，闭合性骨缺损的骨再生明显延迟。拔牙创口的新骨形成无明显差异。与对照组相比，高脂饮食喂养的小鼠拔牙创口的舌侧牙槽嵴高度降低更为明显。
高血脂导致大型闭合性骨缺损的骨再生延迟。虽然拔牙创口较小，在高脂环境下通常可再生，但牙槽嵴高度的明显降低也是未来牙齿修复的一个挑战。
