Nishtar Medical University Multan, Pakistan.
Department of Medicine, Nawab Sir Sadiq Muhammad Khan Abbasi Hospital, Bahawalpur, Pakistan.
Pak J Pharm Sci. 2023 May;36(3(Special)):969-972.
Probiotic bacteria have capacity to bind with heavy metals. The present study was planned to assess the bioremediation potential of probiotic Lactobacillus species isolated from yogurt samples. L. acidophilus and L. plantarum were tested for acidic pH tolerance, bile salt resistance and gastric juice tolerance. The antibiotic susceptibility and antimicrobial activity was also checked. These Lactobacillus species were also evaluated for degradation of lead (Pb) and cadmium (Cd) metals. The results indicated that L. acidophilus and L. plantarum were able to tolerate high acidic pH: 3. both showed significant growth after exposure to stimulated gastric juice from 0 to 24 hours. The significant plate count was observed at different bile salt concentrations (0.1%, 0.3%). The isolates showed resistance for all the tested antibiotics except L. acidophilus showed susceptibility for gentamicin and co-amoxiclave. The isolates depicted no antimicrobial activity against the indicator bacteria. L. acidophilus and L. plantarum were capable of tolerating Cd and Pb. Maximum tolerance and removal were observed for Pb by both Lactobacillus spp. The Cd removal was 11.50 and 3.50% while Pb removal was 42.70 and 35.50% for L. plantarum and L. acidophilus, respectively. In conclusion, L. acidophilus and L. Plantarum have potential for bioremediation of heavy metals.
益生菌具有结合重金属的能力。本研究旨在评估从酸奶样品中分离出的益生菌乳杆菌属的生物修复潜力。测试了嗜酸乳杆菌和植物乳杆菌对酸性 pH 值的耐受性、胆汁盐抗性和胃酸耐受性。还检查了抗生素敏感性和抗菌活性。还评估了这些乳杆菌属对铅（Pb）和镉（Cd）金属的降解能力。结果表明，嗜酸乳杆菌和植物乳杆菌能够耐受高酸性 pH 值：3. 在暴露于 0 至 24 小时的刺激胃液后，均表现出显著的生长。在不同胆汁盐浓度（0.1%、0.3%）下观察到显著的平板计数。除了对庆大霉素和复方新诺明敏感外，分离株对所有测试的抗生素均具有抗性。分离株对指示菌没有抗菌活性。嗜酸乳杆菌和植物乳杆菌能够耐受 Cd 和 Pb。两种乳杆菌属对 Pb 的最大耐受和去除率均观察到。植物乳杆菌和嗜酸乳杆菌对 Cd 的去除率分别为 11.50%和 3.50%，对 Pb 的去除率分别为 42.70%和 35.50%。总之，嗜酸乳杆菌和植物乳杆菌具有重金属生物修复的潜力。
