Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, India.
Department of Biochemistry, Cell Biology and Genetics, College Medicine, American University of Antigua, Cell Biology and Genetics, American University of Antigua, College of Medicine, St. John's, Antigua and Barbuda.
J Biophotonics. 2023 Nov;16(11):e202300021. doi: 10.1002/jbio.202300021. Epub 2023 Sep 2.
The 325 nm-excited autofluorescence spectra from cancerous and normal renal tissues were collected ex vivo biopsy tissue samples, through an optical fiber probe-based system. Noticeable changes in intensity/wavelength were observed in the fluorescence emissions from endogenous fluorophores such as collagen, Nicotinamide adenine dinucleotide (NADH), Vitamin A (retinol), and flavin adenine dinucleotide, in pathological conditions with respect to the normal state. The energy metabolism involved in clear cell renal cell carcinoma (ccRCC) and chromophobe renal cell carcinoma (chRCC) are reflected in the fluorescence emission band at 445 nm due to bound NADH attributed to enhanced oxidative phosphorylation in chRCC and emission at 465 nm contributed by free NADH showing higher glycolytic action in ccRCC. The principal component analysis and one-way ANOVA effectively discriminate ccRCC from chRCC. It is shown that laser induced fluorescence technique with 325 nm excitation can be a suitable technique for optical pathology and in vivo surgical boundary demarcation in renal cell carcinoma.
通过基于光纤探针的系统，从癌症和正常肾组织的活检组织样本中采集了 325nm 激发的自发荧光光谱。在病理性条件下，与正常状态相比，内源性荧光团（如胶原、烟酰胺腺嘌呤二核苷酸（NADH）、维生素 A（视黄醇）和黄素腺嘌呤二核苷酸）的荧光发射强度/波长发生了明显变化。透明细胞肾细胞癌（ccRCC）和嫌色细胞肾细胞癌（chRCC）中的能量代谢反映在 445nm 处的荧光发射带中，这归因于 chRCC 中结合的 NADH 增强了氧化磷酸化，而在 465nm 处的发射则归因于游离 NADH，表明 ccRCC 中的糖酵解作用更高。主成分分析和单因素方差分析有效地将 ccRCC 与 chRCC 区分开来。结果表明，325nm 激发的激光诱导荧光技术可以成为肾细胞癌光学病理学和术中手术边界界定的一种合适技术。
