Medical Laboratory Techniques, Vocational School of Health Services, Toros University, Mersin, Turkey.
Mersin University Advanced Technology Education Research and Application Center, Mersin, Turkey.
Naunyn Schmiedebergs Arch Pharmacol. 2023 Nov;396(11):3327-3335. doi: 10.1007/s00210-023-02669-1. Epub 2023 Aug 17.
Paroxetine is extensively utilized in the management of depressive and anxious conditions. Paroxetine works by increasing serotonin levels in nerve cells in the brain. However, limited information is available regarding the direct effects of paroxetine on macrophage cells. Macrophages are a type of leukocytes involved in the body's immune response, playing a crucial role in combating infections. The impact of paroxetine on macrophages has been explored in research, although a comprehensive understanding is still pending. This study aimed to research the potential of administering paroxetine to J774.2 macrophage cells to stimulate the release of GM-CSF, TNF-α, IL-12p40, and IL-6 cytokines. Additionally, we examined the mechanisms of action of paroxetine on the p38 signaling pathway, which is involved in cytokine production, and the PI3K pathway, which is an important mechanism in intracellular signaling. Our findings revealed that paroxetine induced an inflammatory response in macrophages by promoting cytokine synthesis in a non-lipopolysaccharide (LPS) environment. We observed that paroxetine triggered the inflammatory response through the PI3K signaling pathway while suppressing the p38 signaling pathway.
帕罗西汀被广泛用于治疗抑郁和焦虑症。帕罗西汀通过增加大脑神经细胞中的血清素水平来发挥作用。然而，关于帕罗西汀对巨噬细胞的直接影响的信息有限。巨噬细胞是一种白细胞，参与身体的免疫反应，在对抗感染方面起着至关重要的作用。帕罗西汀对巨噬细胞的影响已经在研究中进行了探索，尽管仍需要全面了解。本研究旨在研究给 J774.2 巨噬细胞施用帕罗西汀以刺激 GM-CSF、TNF-α、IL-12p40 和 IL-6 细胞因子释放的潜力。此外，我们还研究了帕罗西汀对参与细胞因子产生的 p38 信号通路和 PI3K 通路的作用机制，PI3K 通路是细胞内信号的重要机制。我们的研究结果表明，帕罗西汀在非脂多糖（LPS）环境中通过促进细胞因子合成诱导巨噬细胞炎症反应。我们观察到，帕罗西汀通过 PI3K 信号通路触发炎症反应，同时抑制 p38 信号通路。
