Department of Infectious Disease and Global Health, Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, United States of America.
BioMEMS Resource Center, Center for Engineering in Medicine and Surgery, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospitals for Children, Boston, MA, United States of America.
PLoS One. 2023 Aug 17;18(8):e0287997. doi: 10.1371/journal.pone.0287997. eCollection 2023.
We identified a fragment (Domain 3-D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C. hominis and C. parvum anthroponosum. The fragment is highly antigenic and is able to effectively differentiate between zoonotic C. parvum and species/genotypes that infect preferentially humans. D3 detection provides a serological tool to determine whether the source of human cryptosporidiosis is of animal or human origin. We demonstrate this in experimentally challenged piglets, mice, rats, and alpaca. We speculate that the absence of this fragment from the C. hominis and C. parvum anthroponosum gp900 protein may play a key role in their host restriction.
我们鉴定了一个片段（结构域 3-D3），该片段来自人兽共患寄生虫隐孢子虫免疫显性的子孢子表面糖蛋白 gp900，而该片段在人源隐孢子虫和人源优势感染的种/基因型隐孢子虫中是缺失的。该片段具有高度的抗原性，能够有效地将人兽共患的小隐孢子虫与优先感染人类的种/基因型隐孢子虫区分开来。D3 的检测提供了一种血清学工具，以确定人类隐孢子虫病的来源是动物源性还是人源性。我们在实验性感染的仔猪、小鼠、大鼠和羊驼中证明了这一点。我们推测，该片段在人源隐孢子虫和人源优势感染的小隐孢子虫 gp900 蛋白中的缺失可能在其宿主限制中起着关键作用。
