Clinical and Biological Sample Center, Medical Innovation Research Division of Chinese PLA General Hospital, Beijing, China.
Beijing Jinghua Anliang Technology Co., Ltd., Beijing, China.
Biopreserv Biobank. 2024 Apr;22(2):146-156. doi: 10.1089/bio.2022.0219. Epub 2023 Aug 17.
The aim of the study was to store urine samples at different temperatures and humidity levels and analyze common biochemical test results and point-of-care testing (POCT) indicators according to different storage times and evaluate whether the samples should be centrifuged to study the best storage conditions for urine samples. Random midstream urine samples (100 mL) were collected from 10 healthy individuals. A portion of the samples was centrifuged. The remaining samples were not centrifuged and were stored under different temperature and humidity conditions for different periods. We measured urine indicators ([Na+], [K+], [Cl-], gamma-glutamyl transpeptidase [GGT], urea, and creatinine [Cr]) at 2, 4, 24, and 72 hours and 7 and 55 days, and we used POCT to measure myoglobin (Mb) and microalbumin (mAlb) concentrations. Centrifugation of urine samples decreased the measured GGT and increased the measured Mb. In urine samples stored at 4°C and room temperature, electrolyte concentrations were scarcely affected by storage time. After storage at 50°C for 24 hours, the measured [Na] and [Cl] levels changed. Metabolites (urea and Cr) underwent no obvious change across temperatures. GGT did not change during long-term storage at 4°C. The mAlb level changed significantly only after storage at 4°C. When stored at 4°C, Mb changed little within 4 hours. Under humid conditions, [Na] and [Cl] increased significantly after 24 hours, and urea decreased significantly after 7 days of storage. Under dry storage conditions, urinary Cr and GGT decreased, and under humid conditions, these concentrations increased. At high humidity, mAlb increased significantly after 72 hours. Electrolyte and amino acid metabolite concentrations were less affected by storage time at 4°C and room temperature than at other temperatures. Some proteins are sensitive to environmental changes; samples collected for quantification of these proteins can be stored briefly at 4°C after centrifugation. Normal humidity conditions meet most physiological testing requirements.
本研究的目的是在不同温度和湿度条件下储存尿液样本，并根据不同储存时间分析常见生化检测结果和即时检测（POCT）指标，评估样本是否需要离心以研究尿液样本的最佳储存条件。从 10 名健康个体中采集了随机中段尿液（100ml）的一部分进行离心，其余部分不离心，并在不同温度和湿度条件下储存不同时间。我们在 2、4、24 和 72 小时以及 7 和 55 天时测量了尿液指标([Na+]、[K+]、[Cl-]、γ-谷氨酰转肽酶[GGT]、尿素和肌酐[Cr])，并用 POCT 测量了肌红蛋白（Mb）和微量白蛋白（mAlb）浓度。尿液样本离心后，测得的 GGT 降低，Mb 升高。在 4°C 和室温下储存的尿液样本中，储存时间对电解质浓度的影响不大。50°C 储存 24 小时后，测量的[Na]和[Cl]水平发生变化。在不同温度下，代谢物（尿素和 Cr）没有明显变化。GGT 在 4°C 长期储存过程中没有变化。仅在 4°C 储存时，mAlb 水平变化显著。在 4°C 下储存时，Mb 在 4 小时内变化不大。在潮湿条件下，储存 24 小时后[Na]和[Cl]显著增加，储存 7 天后尿素显著减少。在干燥储存条件下，尿 Cr 和 GGT 减少，在潮湿条件下，这些浓度增加。在高湿度下，72 小时后 mAlb 显著增加。在 4°C 和室温下，储存时间对电解质和氨基酸代谢物浓度的影响小于其他温度。一些蛋白质对环境变化敏感；离心后可在 4°C 短暂储存用于定量这些蛋白质的样本。正常湿度条件满足大多数生理测试要求。
