线粒体靶向双光子荧光探针用于神经元和斑马鱼中 Cu 和 ATP 的顺序识别。
Mitochondria-targeting two-photon fluorescent probe for sequential recognition of Cu and ATP in neurons and zebrafish.
机构信息
Shandong Provincial Engineering Research Center of Novel Pharmaceutical Excipients, Sustained and Controlled Release Preparations, Dezhou University, Dezhou 253023, China.
Maternity & Child Care Center of Dezhou, Dezhou 253000, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123260. doi: 10.1016/j.saa.2023.123260. Epub 2023 Aug 12.
Highly active mitochondria play a significant role in neuron function. Cu and ATP levels in mitochondria regulate neuronal mitochondrial activity. However, mitochondrial activity was often evaluated by mitochondrial membrane potential. Less is known about the dynamics of Cu and ATP in mitochondria. Herein, we developed a two-photon fluorescence probe (MP), which provided a determination of mitochondrial ATP and Cu. The fluorescence of MP showed remarkable quenching in the presence of Cu and then gradually recovered in the presence of ATP, which can be used for sequential recognition. MP has high sensitivity to Cu and ATP, with limits of detection (LOD) close to 0.31 nM and 13.6 nM, respectively. Using this useful probe, we monitor the fluctuation of concentrations of Cu and ATP by fluorescence imaging at single neuron and zebrafish.
活性高的线粒体在神经元功能中起着重要作用。线粒体中的铜（Cu）和三磷酸腺苷（ATP）水平调节神经元线粒体活性。然而，线粒体活性通常通过线粒体膜电位来评估。对于线粒体中 Cu 和 ATP 的动态变化知之甚少。在此，我们开发了一种双光子荧光探针（MP），可用于测定线粒体中的 ATP 和 Cu。MP 的荧光在存在 Cu 时表现出明显的猝灭，然后在存在 ATP 时逐渐恢复，可用于顺序识别。MP 对 Cu 和 ATP 具有高灵敏度，检测限（LOD）分别接近 0.31 nM 和 13.6 nM。利用这种有用的探针，我们通过在单个神经元和斑马鱼上进行荧光成像来监测 Cu 和 ATP 浓度的波动。
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