Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Food Chem. 2021 May 1;343:128513. doi: 10.1016/j.foodchem.2020.128513. Epub 2020 Oct 31.
Herein, we developed a turn-on red-emitting fluorescent probe for the sensitive and selective detection of copper ions (Cu) in food samples and living zebrafish. The probe employs a hemicyanine scaffold as the fluorophore and a 2-pyridinecarbonyl group as the recognition receptor and quenching moiety. The 2-pyridinecarbonyl moiety can be specifically cleaved by Cu and results in an approximately 18-fold fluorescence enhancement of the probe, thereby providing a fluorescence turn-on assay for Cu. Additionally, the probe exhibited excellent selectivity, high sensitivity, a broad linear relationship (0.020 to 8.0 μM), and a low limit of detection (4.0 nM, S/N = 3) for Cu. Concomitantly, the probe exhibited satisfactory analytical performance when used with actual food samples. Moreover, the probe could be used for in situ determination of Cu in both living plant tissues and in living zebrafish.
在这里，我们开发了一种用于灵敏和选择性检测食品样品和活体斑马鱼中铜离子（Cu）的开启型红色发射荧光探针。该探针采用半花菁骨架作为荧光团，2-吡啶甲酰基作为识别受体和猝灭部分。2-吡啶甲酰基部分可以被 Cu 特异性切割，导致探针的荧光增强约 18 倍，从而提供了用于 Cu 的荧光开启测定法。此外，该探针表现出优异的选择性、高灵敏度、宽线性关系（0.020 至 8.0 μM）和低检测限（4.0 nM，S/N = 3）用于 Cu。同时，该探针在实际食品样品中的分析性能令人满意。此外，该探针可用于活体植物组织和活体斑马鱼中 Cu 的原位测定。
