Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Department of Immunology, School of Medicine, University of Washington, Seattle, WA, 98109, USA.
Dev Comp Immunol. 2023 Nov;148:104916. doi: 10.1016/j.dci.2023.104916. Epub 2023 Aug 15.
The cytokine interleukin (IL)-22 has been identified in several fish species; however, its functional significance in the gills of these fish species remains unclear. In this study, we analyzed the expression of proinflammatory cytokines, antimicrobial peptides, and IL-22 binding protein in the gills of wild-type and IL-22-knockout (IL-22 KO) medaka under dextran sulfate sodium-induced inflammation. We also produced medaka recombinant IL-22 (rIL-22) and analyzed the expression of immune-related genes in rIL-22-stimulated primary cell cultures from gills. The il1b, il6, tnfa, and hamp genes were significantly upregulated in wild-type gills upon dextran sulfate sodium stimulation compared with the naïve state but not in IL-22 KO gills. il22bp transcripts were barely detectable in the IL-22 KO medaka gills. However, the expression of il1b, il6, hamp, and il22bp was upregulated in rIL-22-stimulated gill cell culture. These results suggest IL-22 could be involved in immune responses through inflammatory cytokine and antimicrobial peptide production in fish gills.
细胞因子白细胞介素（IL）-22 在几种鱼类中被鉴定出来；然而，其在这些鱼类鳃中的功能意义尚不清楚。在这项研究中，我们分析了在葡聚糖硫酸钠诱导的炎症下，野生型和 IL-22 敲除（IL-22 KO）品系斑马鱼鳃中促炎细胞因子、抗菌肽和 IL-22 结合蛋白的表达。我们还产生了斑马鱼重组 IL-22（rIL-22），并分析了 rIL-22 刺激的来自鳃的原代细胞培养物中免疫相关基因的表达。与未刺激状态相比，在葡聚糖硫酸钠刺激下，野生型鱼鳃中 il1b、il6、tnfa 和 hamp 基因的表达显著上调，但在 IL-22 KO 鱼鳃中没有上调。IL-22 KO 斑马鱼鳃中几乎检测不到 il22bp 转录本。然而，在 rIL-22 刺激的鳃细胞培养物中，il1b、il6、hamp 和 il22bp 的表达上调。这些结果表明，IL-22 可能通过鱼类鳃中的炎症细胞因子和抗菌肽产生参与免疫反应。
