Habibi Nazima, Uddin Saif, Behebehani Montaha, Khan Mohd Wasif, Razzack Nasreem Abdul, Shirshikhar Faiz
Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, Kuwait.
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada.
Data Brief. 2023 Jul 28;50:109449. doi: 10.1016/j.dib.2023.109449. eCollection 2023 Oct.
Calanoid copepod populations are being severely affected due to the effects of ocean acidification (OA) and ocean warming (OW). These marine organisms are the most abundant primary consumers contributing significantly in the marine food web. Any effect on the abundance and diversity of copepods due to climate change is likely to have serious implications on the marine ecosystem functioning. Molecular studies that play a vital role in assessing the genetic changes under the influence of environmental imbalances are completely lacking for this species. Here we report the genetic variations in three generations of copepods through transcriptome sequencing. RNA sequencing was performed on an Illumina HiSeq platform employing the 2 × 100 bp paired-end chemistry. Approximately, 10GB of data was obtained for all the samples. The raw sequences were assembled through Trinity 2.6.6 and mined for single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs). MIcroSAtellite Identification Tool (MISA) was used for SSR detection and Primer 3 (v 3.0) was utilized to design short oligonucleotide primers (18-20 mers). A total of 15,222 SSRs were identified and 28,944 primer pairs were designed against these motifs. The transcriptome possessed 413,890 SNPs at a frequency of 2.8 per kb. The newly discovered SSRs and SNPs could act as genetic markers for future studies on genetic diversity and conservation for .
由于海洋酸化(OA)和海洋变暖(OW)的影响,哲水蚤类桡足类种群正受到严重影响。这些海洋生物是最丰富的初级消费者,在海洋食物网中发挥着重要作用。气候变化对桡足类的丰度和多样性产生的任何影响都可能对海洋生态系统功能产生严重影响。对于该物种而言,完全缺乏在评估环境失衡影响下的遗传变化方面发挥关键作用的分子研究。在此,我们通过转录组测序报告了三代桡足类的遗传变异。在Illumina HiSeq平台上采用2×100 bp双末端化学方法进行RNA测序。所有样本大约获得了10GB的数据。原始序列通过Trinity 2.6.6进行组装,并挖掘单核苷酸多态性(SNP)和简单序列重复(SSR)。使用微卫星识别工具(MISA)进行SSR检测,并利用Primer 3(v 3.0)设计短寡核苷酸引物(18 - 20个碱基)。总共鉴定出15,222个SSR,并针对这些基序设计了28,944对引物。转录组在每千碱基频率为2.8时拥有413,890个SNP。新发现的SSR和SNP可作为未来该物种遗传多样性和保护研究的遗传标记。