来自兔胃的类铃蟾肽免疫反应性肽

Physalaemin-like immunoreactive peptides from rabbit stomach.

作者信息

Wilson W E, Harvan D J, Hamm C, Lazarus L H, Klapper D G, Yajima H, Hayashi Y

出版信息

Int J Pept Protein Res. 1986 Jul;28(1):58-66. doi: 10.1111/j.1399-3011.1986.tb03229.x.

DOI:10.1111/j.1399-3011.1986.tb03229.x

Abstract

Two physalaemin (PHY)-like immunoreactive peptides, designated PHLIPs, have been purified from extracts of rabbit stomach tissue. Fast atom bombardment/mass spectrometry (FAB/MS) indicated that the m/z values for the PHLIP protonated molecular ions were 867.419 and 796.4. FAB/tandem MS spectra, coupled with a knowledge of the amino acid composition and the aid of a computerized fragment-matching program, indicated the amino acid sequences to be: (formula; see text) The sequences of PHLIPs-7 and -8 were confirmed with synthetic peptides. The PHY-antiserum cross-reactivity of the PHLIPs reflects homology at amino acid residues 1, 3, 4 and 5 for the mammalian and amphibian residues.

摘要

从兔胃组织提取物中已纯化出两种类似physalaemin（PHY）的免疫反应性肽，命名为PHLIPs。快原子轰击/质谱（FAB/MS）表明，PHLIP质子化分子离子的m/z值分别为867.419和796.4。FAB串联质谱谱图，结合氨基酸组成知识并借助计算机化片段匹配程序，表明氨基酸序列为：（分子式；见正文）PHLIPs - 7和 - 8的序列通过合成肽得到确认。PHLIPs的PHY抗血清交叉反应性反映了哺乳动物和两栖动物残基在氨基酸残基1、3、4和5处的同源性。