Hashemi-Hafshejani Saeideh, Amani Amir, Jafarpour Azami Sanaz, Keshavarz Valian Hossien, Mohebali Mehdi, Salimi Mahboobeh, Lafmejan Pour Hossien Hassani, Shojaee Saeedeh
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2023 Jul;52(7):1495-1503. doi: 10.18502/ijph.v52i7.13252.
infection is caused by , which is an intracellular protozoan parasite. This infection consequently lead various congenital disabilities during pregnancy in patients. Spiramycin (Spi), a macrolide antibiotic, is typically recommended for infection in pregnant women. We aimed to prepare the nanoemulsion of spiramycin (NE-Spi) and to evaluate the activity of this formulation in tachyzoites of , RH strain.
This study was conducted in 2019-2021 at the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. NE-Spi was prepared by spontaneous emulsification. The effects of this nanoemulsion on the viability of cultured cells were measured using MTT assay. To estimate the effects of NE-Spi on tachyzoites of , RH strain, different concentrations of NE-Spi, S-Spi (suspension of spiramycin), and NE (nanoemulsion without any spiramycin) were added to tachyzoites and then stored for 30, 60, 90, 120 min and 24 h in 250 µg/ml concentration at room temperature. Finally, Tachyzoites mortality rates were evaluated by trypan blue staining. Of note, flow cytometry was conducted to confirm the obtained results.
The final particle size of NE-Spi was calculated to be 11.3 nm by DLS and TEM. Thereafter, using MTT assay, in 62.5 µg/ml concentration of NE-Spi, the Vero cells viability was obtained as 82%. The highest mortality rates of tachyzoites of , RH strain were observed at 250 µg/ml concentration and after 120 min of exposure, but it was not significantly different from 24 h of exposure.
NE-Spi has lethal efficacy on RH strain in-vitro.
感染由 引起， 是一种细胞内原生动物寄生虫。这种感染会导致患者在孕期出现各种先天性残疾。螺旋霉素（Spi）是一种大环内酯类抗生素，通常被推荐用于治疗孕妇的 感染。我们旨在制备螺旋霉素纳米乳剂（NE-Spi），并评估该制剂对 RH株速殖子的活性。
本研究于2019年至2021年在伊朗德黑兰医科大学公共卫生学院进行。NE-Spi通过自发乳化法制备。使用MTT法测定该纳米乳剂对培养细胞活力的影响。为评估NE-Spi对 RH株速殖子的影响，将不同浓度的NE-Spi、S-Spi（螺旋霉素混悬液）和NE（不含任何螺旋霉素的纳米乳剂）加入速殖子中，然后在室温下以250μg/ml的浓度储存30、60、90、120分钟和2小时。最后，通过台盼蓝染色评估速殖子死亡率。值得注意的是，进行了流式细胞术以确认所得结果。
通过动态光散射（DLS）和透射电子显微镜（TEM）计算得出NE-Spi的最终粒径为11.3nm。此后，使用MTT法测定，在62.5μg/ml浓度的NE-Spi作用下，Vero细胞活力为82%。在250μg/ml浓度且暴露120分钟后观察到 RH株速殖子的最高死亡率，但与暴露24小时后的死亡率无显著差异。
NE-Spi在体外对 RH株具有致死效力。
