College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, China.
School of Pharmacy, Binzhou Medical University, 346 Guanhai Road, Yantai, Shandong, 264003, China.
Food Funct. 2023 Aug 29;14(17):8008-8017. doi: 10.1039/d3fo01692g.
sp. PCC 7002 has the potential to be used as a new resource of food owing to its nutritional and functional benefits. However, little information has been published regarding the safety of sp. PCC 7002 biomass (SynB). The present study assessed genotoxicity, acute and subchronic toxicity of SynB for the first time. SynB did not show any genotoxicity based on the Ames test, mammalian erythrocyte micronucleus test, and mouse primary spermatocyte chromosome aberration test. SynB administered by gavage in mice at a dose of 10 g per kg body weight did not induce death or toxicity based on the acute toxicity study, indicating the median lethal dose value of SynB was over 10 g per kg body weight. In the 90-day subchronic toxicity study, no treatment-related mortality or clinical sign was noted with SynB at doses of 5 and 10 g per kg body weight in mice, and there was no adverse effect of SynB on food consumption, organ coefficients, blood biochemistry, urinalysis and histopathology. The Non Observed Adverse Effect Level for SynB in female and male mice was not less than 10 g per kg body weight per day based on subchronic toxicity. These results support the safe use of SynB as a new food raw material.
sp. PCC 7002 由于其营养价值和功能优势，有望成为一种新的食品资源。然而，关于 sp. PCC 7002 生物质（SynB）的安全性的信息却很少。本研究首次评估了 SynB 的遗传毒性、急性毒性和亚慢性毒性。基于 Ames 试验、哺乳动物红细胞微核试验和小鼠初级精母细胞染色体畸变试验，SynB 没有表现出任何遗传毒性。在急性毒性研究中，以 10g/kg 体重的剂量经灌胃给予小鼠 SynB 不会引起死亡或毒性，表明 SynB 的半数致死剂量值超过 10g/kg 体重。在 90 天亚慢性毒性研究中，以 5 和 10g/kg 体重的剂量给予小鼠 SynB 时，未观察到与治疗相关的死亡率或临床症状，SynB 对食物消耗、器官系数、血液生化学、尿液分析和组织病理学没有不良影响。基于亚慢性毒性，SynB 对雌性和雄性小鼠的无观察不良效应水平不低于 10g/kg 体重/天。这些结果支持将 SynB 作为一种新的食品原料安全使用。
