Department of Civil Engineering, Nagoya Institute of Technology, Nagoya, Japan.
Department of Physical Science and Engineering, Nagoya Institute of Technology, Nagoya, Japan.
Environ Microbiol Rep. 2023 Dec;15(6):557-567. doi: 10.1111/1758-2229.13192. Epub 2023 Aug 18.
Advances in many isolation studies have revealed that pure Dehalococcoides grow stably, although the large-scale pure cultivation of Dehalococcoides has yet to be established. In this study, 7 L-culturing of Dehalococcoides mccartyi strain NIT01 was first performed using vessels made of glass and stainless alloy SUS304. All batches cultured in the glass vessel successfully dechlorinated >95% of 1 mM trichloroethene (TCE) to ethene (ETH), whereas only 5 out of 13 batches cultured in the SUS304 vessel did the same. The difference in dechlorination efficiency suggested the possible inhibition of dechlorination by SUS304. Also, the strain NIT01 showed long delays in dechlorination with pieces of SUS316, steel, and a repeatedly used SUS304, but not with titanium. The repeatedly used SUS304 cracked and increased the Fe concentration to ≥76 μM. Dechlorination by this strain was also inhibited with ≥1000 μM Fe and ≥23 μM Cr but not with ≤100 μM Ni , suggesting that Cr eluted from solid stainless alloys inhibited the dechlorination. Culturing in a titanium vessel instead of a stainless alloy showed the complete dechlorination of 1 mM TCE within 12-28 days with a growth yield of 2.7 × 10 cells/μmol-released Cl , even after repeating use of the vessels six times.
在许多隔离研究的进展表明，纯脱氯菌属稳定生长，尽管尚未建立大规模的纯培养脱氯菌属。在这项研究中，首次使用玻璃和不锈钢合金 SUS304 制成的容器对 Dehalococcoides mccartyi 菌株 NIT01 进行了 7L 培养。所有在玻璃容器中培养的批次均成功地将 1mM 三氯乙烯（TCE）脱氯>95%至乙烯（ETH），而在 SUS304 容器中培养的 13 批中只有 5 批达到了相同的效果。脱氯效率的差异表明 SUS304 可能抑制了脱氯作用。此外，该菌株 NIT01 与 SUS316、钢和重复使用的 SUS304 接触时，脱氯时间明显延长，但与钛则没有。重复使用的 SUS304 出现裂纹并将铁浓度增加到≥76μM。该菌株的脱氯作用也受到≥1000μM Fe 和≥23μM Cr 的抑制，但不受≤100μM Ni 的抑制，表明从固体不锈钢合金中洗脱的 Cr 抑制了脱氯作用。在钛容器中培养而不是在不锈钢合金中培养，即使在重复使用容器六次后，也能在 12-28 天内将 1mM TCE 完全脱氯，释放的 Cl 的细胞生长产率为 2.7×10 cells/μmol。
